ArticleProceedings of the National Academy of Sciences of the United States of America2016
Globin X is a six-coordinate globin that reduces nitrite to nitric oxide in fish red blood cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 56 citations in OpenAlex.
- Effects of Oral Sodium Nitrite on Blood Pressure, Insulin Sensitivity, and Intima-Media Arterial Thickening in Adults With Hypertension and Metabolic Syndrome.Hypertension (Dallas, Tex. : 1979) · 2020Trial
- Artificial Oxygen Carriers: Lessons From Hemoglobin-Based Oxygen Carrier Clinical Trials and Current Development Efforts.Shock (Augusta, Ga.) · 2026Review
- Measurement of nitric oxide production in mouse tracheal epithelial cell cultures differentiated at the air liquid interface.bioRxiv : the preprint server for biology · 2025Article
- The circularly permuted globin domain of androglobin exhibits atypical heme stabilization and nitric oxide interaction.Chemical science · 2024Article
- Cytoglobin regulates NO-dependent cilia motility and organ laterality during development.Nature communications · 2023Article
- Insights into the function of cytoglobin.Biochemical Society transactions · 2023Review
- Nitric Oxide Function and Nitric Oxide Synthase Evolution in Aquatic Chordates.International journal of molecular sciences · 2023Review
- Article
- Cardiac Hypoxia Tolerance in Fish: From Functional Responses to Cell Signals.International journal of molecular sciences · 2023Review
- Review
- Nitric Oxide Production and Regulation in the Teleost Cardiovascular System.Antioxidants (Basel, Switzerland) · 2022Review
- Shaping the cardiac response to hypoxia: NO and its partners in teleost fish.Current research in physiology · 2022Article
- Whole-Genome Duplications and the Diversification of the Globin-X Genes of Vertebrates.Genome biology and evolution · 2021Article
- Lessons from the post-genomic era: Globin diversity beyond oxygen binding and transport.Redox biology · 2020Review
- BMP10-mediated ALK1 signaling is continuously required for vascular development and maintenance.Angiogenesis · 2020Article
- Nitrite Improves Heart Regeneration in Zebrafish.Antioxidants & redox signaling · 2020Article
- Carbonic anhydrase II does not regulate nitrite-dependent nitric oxide formation and vasodilation.British journal of pharmacology · 2020Article
- Article
- The Zebrafish CytochromeBiochemistry · 2019Article
- Nitrite and nitrate chemical biology and signalling.British journal of pharmacology · 2019Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
The discovery of novel globins in diverse organisms has stimulated intense interest in their evolved function, beyond oxygen binding. Globin X (GbX) is a protein found in fish, amphibians, and reptiles that diverged from a common ancestor of mammalian hemoglobins and myoglobins. Like mammalian neuroglobin, GbX was first designated as a neuronal globin in fish and exhibits six-coordinate heme geometry, suggesting a role in intracellular electron transfer reactions rather than oxygen binding. Here, we report that GbX to our knowledge is the first six-coordinate globin and the first globin protein apart from hemoglobin, found in vertebrate RBCs. GbX is present in fish erythrocytes and exhibits a nitrite reduction rate up to 200-fold faster than human hemoglobin and up to 50-fold higher than neuroglobin or cytoglobin. Deoxygenated GbX reduces nitrite to form nitric oxide (NO) and potently inhibits platelet activation in vitro, to a greater extent than hemoglobin. Fish RBCs also reduce nitrite to NO and inhibit platelet activation to a greater extent than human RBCs, whereas GbX knockdown inhibits this nitrite-dependent NO signaling. The description of a novel, six-coordinate globin in RBCs with dominant electron transfer and nitrite reduction functionality provides new insights into the evolved signaling properties of ancestral heme-globins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.