Evidence map›Paper›PMID 27406385›Full record

ArticleFish physiology and biochemistry2016

Characterization of a vasa homolog in the brown-marbled grouper (Epinephelus fuscoguttatus) and its expression in gonad and germ cells during larval development.

Surintorn Boonanuntanasarn, Paiboon Bunlipatanon, Kensuke Ichida, Kirana Yoohat, Ornkanya Mengyu, Samart Detsathit, Ryosuke Yazawa, Goro Yoshizaki

Abstract read
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In one paragraph

Article in Fish physiology and biochemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.9field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 15 citations in OpenAlex.

  1. Characterization ofAnimals : an open access journal from MDPI · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Surintorn BoonanuntanasarnSchool of Animal Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Muang, Nakhon Ratchasima, 30000, Thailand. surinton@sut.ac.th.
Paiboon BunlipatanonKrabi Coastal Fisheries Research and Development Center, 141 Moo 6, Saithai, Muang, Krabi, 81000, Thailand.
Kensuke IchidaDepartment of Marine Biosciences, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-Ku, Tokyo, 108-8477, Japan.
Kirana YoohatSchool of Animal Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Muang, Nakhon Ratchasima, 30000, Thailand.
Ornkanya MengyuKrabi Coastal Fisheries Research and Development Center, 141 Moo 6, Saithai, Muang, Krabi, 81000, Thailand.
Samart DetsathitKrabi Coastal Fisheries Research and Development Center, 141 Moo 6, Saithai, Muang, Krabi, 81000, Thailand.
Ryosuke YazawaDepartment of Marine Biosciences, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-Ku, Tokyo, 108-8477, Japan.
Goro YoshizakiDepartment of Marine Biosciences, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-Ku, Tokyo, 108-8477, Japan.
Tokyo University of Marine Science and Technology · JPSuranaree University of Technology · TH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The vasa gene is specifically expressed in the germ cell lineage, and its expression has been used to study germline development in many organisms, including fishes. In this study, we cloned and characterized vasa as Efu-vasa in the brown-marbled grouper (Epinephelus fuscoguttatus). Efu-vasa contained predicted regions that shared consensus motifs with the vasa family in teleosts, including arginine- and glycine-rich repeats, ATPase motifs, and a DEAD box. Phylogenetic-tree construction using various DEAD-box proteins confirmed that Efu-vasa was clustered in the vasa family. Efu-vasa mRNA was detectable only in gonads, by reverse transcription polymerase chain reaction. Primordial germ cells (PGCs) during early gonad development in larvae were characterized by histological examination and in situ hybridization using an Efu-vasa antisense probe. Migrating PGCs were found in larvae at 9-21 days post-hatching, and rapid proliferation of PGCs was initiated in 36 days post-hatching. These findings provide a valuable basis for optimizing the developmental stages for germ cell transplantation in order to produce surrogate broodstock, which may help in the production of larvae of large and endangered grouper species.

Indexed as

PerciformesAmino Acid SequenceAnimalsBase SequenceCell MovementCloning, MolecularDEAD-box RNA HelicasesDNA, ComplementaryFemaleFish ProteinsGene Expression Regulation, DevelopmentalGerm CellsLarvaMaleOvaryPhylogenyDEAD-box RNA HelicasesDNA, ComplementaryFish ProteinsRNA, MessengerBrown-marbled grouperEpinephelus fuscoguttatusPGCPrimordial germ cell migrationVasaVasa molecular characterization

Identifiers

PMID27406385
OpenAlexW2466631713

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.