ArticlePlant physiology2016
Long-Term Growth of Moss in Microfluidic Devices Enables Subcellular Studies in Development.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 67 citations in OpenAlex.
- Morphogenesis of moss leaf-like organs through variations in deeply shared developmental principles.Science advances · 2026Article
- TON1 and FASS are required to recruit myosin VIII, a cell division guidance factor.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Microfluidic Interrogation of Chitin-Induced Calcium Oscillations in the MossPlants (Basel, Switzerland) · 2026Article
- CRISPR/Cas9 targeted genetic screening in Physcomitrella identifies novel cell division genes.Frontiers in plant science · 2025Article
- An alternate route for cellulose microfibril biosynthesis in plants.Science advances · 2024Article
- Cellulose synthase-like D movement in the plasma membrane requires enzymatic activity.The Journal of cell biology · 2023Article
- Intracellular connections between basal bodies promote the coordinated behavior of motile cilia.Molecular biology of the cell · 2022Article
- COPII Sec23 proteins form isoform-specific endoplasmic reticulum exit sites with differential effects on polarized growth.The Plant cell · 2022Article
- Bioimaging tools move plant physiology studies forward.Frontiers in plant science · 2022Article
- Physcomitrium patens Protoplasting and Protoplast Transfection.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Microfluidics-Based Bioassays and Imaging of Plant Cells.Plant & cell physiology · 2021Review
- Article
- Leaf Morphogenesis: Insights From the MossFrontiers in plant science · 2021Review
- A Fully Functional ROP Fluorescent Fusion Protein Reveals Roles for This GTPase in Subcellular and Tissue-Level Patterning.The Plant cell · 2020Article
- The MossThe Plant cell · 2020Review
- Article
- Microfluidics for interrogating live intact tissues.Microsystems & nanoengineering · 2020Review
- A versatile microfluidic device for highly inclined thin illumination microscopy in the moss Physcomitrella patens.Scientific reports · 2019Article
- Light-inducible activation of cell cycle progression in Xenopus egg extracts under microfluidic confinement.Lab on a chip · 2019Article
- Efficient and modular CRISPR-Cas9 vector system forPlant direct · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Key developmental processes that occur on the subcellular and cellular level or occur in occluded tissues are difficult to access, let alone image and analyze. Recently, culturing living samples within polydimethylsiloxane (PDMS) microfluidic devices has facilitated the study of hard-to-reach developmental events. Here, we show that an early diverging land plant, Physcomitrella patens, can be continuously cultured within PDMS microfluidic chambers. Because the PDMS chambers are bonded to a coverslip, it is possible to image P. patens development at high resolution over long time periods. Using PDMS chambers, we report that wild-type protonemal tissue grows at the same rate as previously reported for growth on solid medium. Using long-term imaging, we highlight key developmental events, demonstrate compatibility with high-resolution confocal microscopy, and obtain growth rates for a slow-growing mutant. By coupling the powerful genetic tools available to P. patens with long-term growth and imaging provided by PDMS microfluidic chambers, we demonstrate the capability to study cellular and subcellular developmental events in plants directly and in real time.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.