ArticleAnalytical and bioanalytical chemistry2016
Metabolomics profiling of the free and total oxidised lipids in urine by LC-MS/MS: application in patients with rheumatoid arthritis.
Article in Analytical and bioanalytical chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Maternal urinary metabolomic signatures preceding spontaneous preterm birth: A pilot study.Scientific reports · 2025Article
- Species-specific optimization of oxylipin ionization in LC-MS: a design of experiments approach to improve sensitivity.Analytical and bioanalytical chemistry · 2025Article
- The potential role of ischaemia-reperfusion injury in chronic, relapsing diseases such as rheumatoid arthritis, Long COVID, and ME/CFS: evidence, mechanisms, and therapeutic implications.The Biochemical journal · 2022Review
- Maternal prebiotic supplementation impacts colitis development in offspring mice.Frontiers in nutrition · 2022Article
- Metabolomics in rheumatoid arthritis: Advances and review.Frontiers in immunology · 2022Review
- A transcriptome-wide association study identifies novel blood-based gene biomarker candidates for Alzheimer's disease risk.Human molecular genetics · 2021Article
- Mass spectrometry-based study defines the human urine nitrolipidome.Free radical biology & medicine · 2021Article
- Metabolic Enzyme Triosephosphate Isomerase 1 and Nicotinamide Phosphoribosyltransferase, Two Independent Inflammatory Indicators in Rheumatoid Arthritis: Evidences From Collagen-Induced Arthritis and Clinical Samples.Frontiers in immunology · 2021Article
- Article
- Quantitative metabolic profiling of urinary eicosanoids for clinical phenotyping.Journal of lipid research · 2019Article
- Mass Spectrometry in Advancement of Redox Precision Medicine.Advances in experimental medicine and biology · 2019Review
- Duodenal L cell density correlates with features of metabolic syndrome and plasma metabolites.Endocrine connections · 2018Article
- Development and application of a UHPLC-MS/MS metabolomics based comprehensive systemic and tissue-specific screening method for inflammatory, oxidative and nitrosative stress.Analytical and bioanalytical chemistry · 2018Article
- Nitrolipids in kidney physiology and disease.Nitric oxide : biology and chemistry · 2018Review
- Simultaneous LC-MS/MS analysis of eicosanoids and related metabolites in human serum, sputum and BALF.Biomedical chromatography : BMC · 2018Article
- A metabolomics study of Qiliqiangxin in a rat model of heart failure: a reverse pharmacology approach.Scientific reports · 2018Article
- Differential toxicity of arsenic on renal oxidative damage and urinary metabolic profiles in normal and diabetic mice.Environmental science and pollution research international · 2017Article
- Ultra-weak photon emission as a dynamic tool for monitoring oxidative stress metabolism.Scientific reports · 2017Article
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Corrections and comments
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Authors and funding
10 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oxidised lipids, covering enzymatic and auto-oxidation-synthesised mediators, are important signalling metabolites in inflammation while also providing a readout for oxidative stress, both of which are prominent physiological processes in a plethora of diseases. Excretion of these metabolites via urine is enhanced through the phase-II conjugation with glucuronic acid, resulting in increased hydrophilicity of these lipid mediators. Here, we developed a bovine liver-β-glucuronidase hydrolysing sample preparation method, using liquid chromatography coupled to tandem mass spectrometry to analyse the total urinary oxidised lipid profile including the prostaglandins, isoprostanes, dihydroxy-fatty acids, hydroxy-fatty acids and the nitro-fatty acids. Our method detected more than 70 oxidised lipids biosynthesised from two non-enzymatic and three enzymatic pathways in urine samples. The total oxidised lipid profiling method was developed and validated for human urine and was demonstrated for urine samples from patients with rheumatoid arthritis. Pro-inflammatory mediators PGF2α and PGF3α and oxidative stress markers iPF2α- IV, 11-HETE and 14-HDoHE were positively associated with improvement of disease activity score. Furthermore, the anti-inflammatory nitro-fatty acids were negatively associated with baseline disease activity. In conclusion, the developed methodology expands the current metabolic profiling of oxidised lipids in urine, and its application will enhance our understanding of the role these bioactive metabolites play in health and disease.
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