ArticleGenome research2016
Novel features of telomere biology revealed by the absence of telomeric DNA methylation.
Article in Genome research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Telomeres: an organized string linking plants and mammals.Biology direct · 2024Review
- Epigenetic nature of Arabidopsis thaliana telomeres.Plant physiology · 2023Article
- Low guanine content and biased nucleotide distribution in vertebrate mtDNA can cause overestimation of non-CpG methylation.NAR genomics and bioinformatics · 2022Article
- A complex network of interactions governs DNA methylation at telomeric regions.Nucleic acids research · 2022Article
- Relative Telomere Length and Telomerase Reverse Transcriptase (TERT) Expression Are Associated with Age in Almond (Plants (Basel, Switzerland) · 2021Article
- Distinct Responses of Arabidopsis Telomeres and Transposable Elements to Zebularine Exposure.International journal of molecular sciences · 2021Article
- Standardization of DNA amount for bisulfite conversion for analyzing the methylation status of LINE-1 in lung cancer.PloS one · 2021Article
- Article
- The epigenetic regulation of centromeres and telomeres in plants and animals.Comparative cytogenetics · 2020Review
- Article
- Epigenetic aspects of telomeric chromatin in Arabidopsis thaliana.BMB reports · 2019Review
- High-resolution DNA methylome reveals that demethylation enhances adaptability to continuous cropping comprehensive stress in soybean.BMC plant biology · 2019Article
- Epigenetic features of human telomeres.Nucleic acids research · 2018Article
- DDM1 guards against telomere truncation in Arabidopsis.Plant cell reports · 2018Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Cytosine methylation regulates the length and stability of telomeres, which can affect a wide variety of biological features, including cell differentiation, development, or illness. Although it is well established that subtelomeric regions are methylated, the presence of methylated cytosines at telomeres has remained controversial. Here, we have analyzed multiple bisulfite sequencing studies to address the methylation status of Arabidopsis thaliana telomeres. We found that the levels of estimated telomeric DNA methylation varied among studies. Interestingly, we estimated higher levels of telomeric DNA methylation in studies that produced C-rich telomeric strands with lower efficiency. However, these high methylation estimates arose due to experimental limitations of the bisulfite technique. We found a similar phenomenon for mitochondrial DNA: The levels of mitochondrial DNA methylation detected were higher in experiments with lower mitochondrial read production efficiencies. Based on experiments with high telomeric C-rich strand production efficiencies, we concluded that Arabidopsis telomeres are not methylated, which was confirmed by methylation-dependent restriction enzyme analyses. Thus, our studies indicate that telomeres are refractory to de novo DNA methylation by the RNA-directed DNA methylation machinery. This result, together with previously reported data, reveals that subtelomeric DNA methylation controls the homeostasis of telomere length.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.