Evidence map›Paper›PMID 27405639›Full record

ArticleArthritis research & therapy2016

Periodontal bacterial colonization in synovial tissues exacerbates collagen-induced arthritis in B10.RIII mice.

Sasanka Chukkapalli, Mercedes Rivera-Kweh, Prashasnika Gehlot, Irina Velsko, Indraneel Bhattacharyya, S John Calise, Minoru Satoh, Edward K L Chan, Joseph Holoshitz, Lakshmyya Kesavalu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Arthritis research & therapy, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
3.9field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 52 citations in OpenAlex.

  1. Trial
  2. Article
  3. Review
  4. Review
  5. Review
  6. Review
  7. Review
  8. Review
  9. Review
  10. Unique miRomics Expression Profiles inInternational journal of molecular sciences · 2023
    Article
  11. Review
  12. The oral microbiome in autoimmune diseases: friend or foe?Journal of translational medicine · 2023
    Review
  13. Article
  14. Specific microRNA Signature Kinetics inInternational journal of molecular sciences · 2023
    Article
  15. Review
  16. Anti-Citrullinated Peptide Antibodies Control OralInternational journal of molecular sciences · 2022
    Article
  17. Review
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Sasanka ChukkapalliDepartment of Periodontology and Oral Biology, College of Dentistry, University of Florida Gainesville, Gainesville, FL, 32610, USA.
Mercedes Rivera-KwehDepartment of Periodontology and Oral Biology, College of Dentistry, University of Florida Gainesville, Gainesville, FL, 32610, USA.
Prashasnika GehlotDepartment of Internal Medicine, University of Michigan School of Medicine, Ann Arbor, Michigan, USA.
Irina VelskoDepartment of Periodontology and Oral Biology, College of Dentistry, University of Florida Gainesville, Gainesville, FL, 32610, USA.
Indraneel BhattacharyyaDepartment of Oral & Maxillofacial Diagnostic Sciences, College of Dentistry, University of Florida, Gainesville, Florida, USA.
S John CaliseDepartments of Oral Biology, College of Dentistry, University of Florida, Gainesville, Florida, USA.
Minoru SatohDivision of Rheumatology and Clinical Immunology, College of Medicine, University of Florida, Gainesville, Florida, USA.
Edward K L ChanDepartments of Oral Biology, College of Dentistry, University of Florida, Gainesville, Florida, USA.
Joseph HoloshitzDepartment of Internal Medicine, University of Michigan School of Medicine, Ann Arbor, Michigan, USA.
Lakshmyya KesavaluDepartment of Periodontology and Oral Biology, College of Dentistry, University of Florida Gainesville, Gainesville, FL, 32610, USA. kesavalu@dental.ufl.edu.
University of Florida · USUniversity of Michigan–Ann Arbor · USUniversity of Occupational and Environmental Health Japan · JP

Funding

Immune Modulating HLA-Coded Ligands in Rheumatoid ArthritisR01AR059085 · NIAMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HOLOSHITZ, JOSEPH · 2011 to 2020
$3.8M
Periodontal Pathogens and Cardiovascular DiseaseR01DE020820 · NIDCR · UNIVERSITY OF FLORIDA · PI LAKSHMYYA, KESAVALU NAIDU · 2011 to 2015
$1.8M
A novel bone-destructive pathway in periodontal diseaseR21DE023845 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HOLOSHITZ, JOSEPH · 2014 to 2015
$427k
NIAMS NIH HHS R01 AR059085NIDCR NIH HHS R01 DE020820NIDCR NIH HHS R21 DE023845
6 · The paper itself

Abstract

backgroundIt has been previously hypothesized that oral microbes may be an etiological link between rheumatoid arthritis (RA) and periodontal disease. However, the mechanistic basis of this association is incompletely understood. Here, we investigated the role of periodontal bacteria in induction of joint inflammation in collagen-induced arthritis (CIA) in B10.RIII mice.

methodsCIA-prone B10.RIII mice were infected orally with a polybacterial mixture of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia for 24 weeks before induction of CIA. The ability of polybacterial mixture to colonize the periodontium and induce systemic response, horizontal alveolar bone resorption in infected B10.RIII mice was investigated. Arthritis incidence, severity of joint inflammation, pannus formation, skeletal damage, hematogenous dissemination of the infection, matrix metalloproteinase 3 (MMP3) levels, and interleukin-17 expression levels were evaluated.

resultsB10.RIII mice had gingival colonization with all three bacteria, higher levels of anti-bacterial immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies, significant alveolar bone resorption, and hematogenous dissemination of P. gingivalis to synovial joints. Infected B10.RIII mice had more severe arthritis, and higher serum matrix metalloproteinase 3 levels and activity. Histopathological analysis showed increased inflammatory cell infiltration, destruction of articular cartilage, erosions, and pannus formation. Additionally, involved joints showed had expression levels of interleukin-17.

conclusionThese findings demonstrate that physical presence of periodontal bacteria in synovial joints of B10.RIII mice with collagen-induced arthritis is associated with arthritis exacerbation, and support the hypothesis that oral bacteria, specifically P. gingivalis, play a significant role in augmenting autoimmune arthritis due to their intravascular dissemination to the joints.

Indexed as

AnimalsArthritis, ExperimentalMicePeriodontitisPeriodontiumSynovial MembraneB10.RIII miceCollagen-induced arthritisPeriodontal bacteriaPeriodontal diseasePorphyromonas gingivalisRheumatoid arthritis

Identifiers

PMID27405639
PMCPMC4942913
OpenAlexW2474407366

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.