Evidence map›Paper›PMID 27405609›Full record

ArticleBMC complementary and alternative medicine2016

Characterization and evaluation of antibacterial and antiproliferative activities of crude protein extracts isolated from the seed of Ricinus communis in Bangladesh.

M Abdulla Al-Mamun, Zerin Akter, Md Josim Uddin, K M K B Ferdaus, K M F Hoque, Z Ferdousi, M Abu Reza

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC complementary and alternative medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed, 1 pooled it
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

M Abdulla Al-MamunDepartment of Genetic Engineering and Biotechnology, Protein Science Lab, University of Rajshahi, Rajshahi, 6205, Bangladesh.
Zerin AkterDepartment of Genetic Engineering and Biotechnology, Protein Science Lab, University of Rajshahi, Rajshahi, 6205, Bangladesh.
Md Josim UddinDepartment of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, Chittagong, 4203, Bangladesh.
K M K B FerdausDepartment of Genetic Engineering and Biotechnology, Protein Science Lab, University of Rajshahi, Rajshahi, 6205, Bangladesh.
K M F HoqueDepartment of Genetic Engineering and Biotechnology, Protein Science Lab, University of Rajshahi, Rajshahi, 6205, Bangladesh.
Z FerdousiDepartment of Genetic Engineering and Biotechnology, Protein Science Lab, University of Rajshahi, Rajshahi, 6205, Bangladesh.
M Abu RezaDepartment of Genetic Engineering and Biotechnology, Protein Science Lab, University of Rajshahi, Rajshahi, 6205, Bangladesh. reza.gen@ru.ac.bd.
University of Rajshahi · BDInternational Islamic University Chittagong · BD

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundRicinus communis (Euphorbiaceae) has previously been reported to possess analgesic, antihistamine, antioxidant and anti-inflammatory activities. This study was designed for isolation, characterization and evaluation of antibacterial and anti-proliferative activities of R. communis seed protein.

methodsThe concentration and molecular weight of R. communis seed protein were estimated by SDS-PAGE and spectrophotometric analysis, respectively. Lectin activity was evaluated by hemagglutination assay on mice blood. In vitro susceptibility of four human pathogenic bacteria including Escherichia coli, Pseudomonas aeruginosa, Enterobacter aerogenes and Staphylococcus aureus was detected using disk diffusion assay, and minimum inhibitory concentration (MIC) value was determined using micro-dilution method. A total of twenty four Swiss albino mice containing Ehrlich's ascites carcinoma (EAC) cells were treated with the crude protein of R. communis at 50 and 100 μg/ml/d/mouse for 6 days. Growth inhibitory activity of R. communis seed protein on EAC cells was determined by haemocytometer counting using trypan blue dye and DAPI (4΄,6-diamidino-2-phenylindole) staining was used to assess apoptotic cells.

resultsThe protein concentration of six R. communis (castor) varieties ranged between 21-35 mg/ml and molecular weight between 14-200 kDa. Castor protein agglutinated mice blood at 3.125 μg/wall. The seed protein shows considerable antimicrobial activity against E. coli, P. aeruginosa and S. aureus, exhibiting MIC values of 250, 125 and 62.5 μg/ml, respectively. Administration of seed protein led to 54 % growth inhibition of EAC cells at 100 μg/ml. DAPI staining indicates marked features of apoptosis including condensation of cytoplasm, nuclear fragmentation and aggregation of apoptotic bodies etc.

conclusionOur study suggests that the lectin rich R. communis seed protein has strong antibacterial and anticancer activities.

Indexed as

AnimalsAnti-Bacterial AgentsAntineoplastic AgentsApoptosisBangladeshCell ProliferationMiceMicrobial Sensitivity TestsNeoplasms, ExperimentalPlant ExtractsPlant ProteinsRicinus communisSeedsAnti-Bacterial AgentsAntineoplastic AgentsPlant ExtractsPlant ProteinsApoptosisCastor beanDisc diffusionEAC cellsLectinMICMiceProtein

Identifiers

PMID27405609
PMCPMC4942971
OpenAlexW2466571769

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.