ArticleJournal of visualized experiments : JoVE2016
Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages.
Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed.
- Trial
- Csep1P protein from Campylobacter concisus induces a chemokine-dominant inflammatory state in macrophages and enhances proinflammatory response to gut bacteria.PLoS pathogens · 2026Article
- Comparison of four culture protocols for differentiating bovine peripheral blood mononuclear cells into macrophages.Frontiers in veterinary science · 2026Article
- A streamlined and comprehensive protocol for the generation and multi-omic analysis of human monocyte-derived macrophages.BMC biotechnology · 2025Article
- Palmitic acid reduces LDLR-dependent uptake of macrophage-derived extracellular vesicles by hepatoma cells.Non-coding RNA research · 2025Article
- Chemoresistant tumor cell secretome potentiates immune suppression in triple negative breast cancer.Breast cancer research : BCR · 2025Article
- Role of Macrophage Phagocytosis as Predictive Marker of the Prevalence of Coronary Heart Disease and Acute Coronary Syndromes.Cardiovascular therapeutics · 2025Article
- Discovery and functional characterization of LncRNAs associated with inflammation and macrophage activation.Methods (San Diego, Calif.) · 2024Review
- NLRP3 Cys126 palmitoylation by ZDHHC7 promotes inflammasome activation.Cell reports · 2024Article
- Single-cell profiling and zebrafish avatars reveal LGALS1 as immunomodulating target in glioblastoma.EMBO molecular medicine · 2023Article
- AXL-initiated paracrine activation of pSTAT3 enhances mesenchymal and vasculogenic supportive features of tumor-associated macrophages.Cell reports · 2023Article
- Hydroxyproline metabolism enhances IFN-γ-induced PD-L1 expression and inhibits autophagic flux.Cell chemical biology · 2023Article
- A novel cysteine protease inhibitor in Baylisascaris schroederi migratory larvae regulates inflammasome activation through the TLR4-ROS-NLRP3 pathway.Parasites & vectors · 2022Article
- Targeting Immunosuppressive Tumor-Associated Macrophages Using Innate T Cells for Enhanced Antitumor Reactivity.Cancers · 2022Article
- An Ex Vivo 3D Tumor Microenvironment-Mimicry Culture to Study TAM Modulation of Cancer Immunotherapy.Cells · 2022Article
- Targeting UDP-α-d-glucose 6-dehydrogenase alters the CNS tumor immune microenvironment and inhibits glioblastoma growth.Genes & diseases · 2022Article
- TMT-based quantitative proteomic profiling of human monocyte-derived macrophages and foam cells.Proteome science · 2022Article
- Characterization of a novel cysteine protease inhibitor inFrontiers in immunology · 2022Article
- Monoallelic IDH1 R132H Mutation Mediates Glioma Cell Response to Anticancer Therapies via Induction of Senescence.Molecular cancer research : MCR · 2021Article
- Mutant IDH1 promotes phagocytic function of microglia/macrophages in gliomas by downregulating ICAM1.Cancer letters · 2021Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
A protocol is presented for cell culture of macrophage colony-stimulating factor (M-CSF) differentiated human monocyte-derived macrophages. For initiation of experiments, fresh or frozen monocytes are cultured in flasks for 1 week with M-CSF to induce their differentiation into macrophages. Then, the macrophages can be harvested and seeded into culture wells at required cell densities for carrying out experiments. The use of defined numbers of macrophages rather than defined numbers of monocytes to initiate macrophage cultures for experiments yields macrophage cultures in which the desired cell density can be more consistently attained. Use of cryopreserved monocytes reduces dependency on donor availability and produces more homogeneous macrophage cultures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.