ArticleScientific reports2016
Highly sensitive detection of lipopolysaccharides using an aptasensor based on hybridization chain reaction.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 53 citations in OpenAlex.
- Lipopolysaccharide-Specific Aptasensor: A Spectroscopic Approach for Identifying Gram-Negative Bacteria.Analytical science advances · 2026Article
- Fluorescence Method-Based Nonlinear Hybridization Chain Reaction for Highly Sensitive and Specific Detection of HER2.International journal of analytical chemistry · 2026Article
- An optical aptasensor for real-time quantification of endotoxin: From ensemble to single-molecule resolution.Science advances · 2023Article
- Environmental protection based on the nanobiosensing of bacterial lipopolysaccharides (LPSs): material and method overview.RSC advances · 2022Review
- Review
- Design and Synthesis of Lipopolysaccharide-Binding Antimicrobial Peptides Based on Truncated Rabbit and Human CAP18 Peptides and Evaluation of Their Action Mechanism.Probiotics and antimicrobial proteins · 2020Article
- Recent Progress in the Identification of Aptamers Against Bacterial Origins and Their Diagnostic Applications.International journal of molecular sciences · 2020Review
- Chitosan Stabilized Silver Nanoparticles for the Electrochemical Detection of Lipopolysaccharide: A Facile Biosensing Approach for Gram-Negative Bacteria.Micromachines · 2020Article
- Sensors, Biosensors, and Analytical Technologies for Aquaculture Water Quality.Research (Washington, D.C.) · 2020Review
- Application of Aptamer-Based Biosensor for Rapid Detection of PathogenicSensors (Basel, Switzerland) · 2018Review
- Nucleic acid aptamer-based methods for diagnosis of infections.Biosensors & bioelectronics · 2018Review
- Ultrasensitive colorimetric detection of circulating tumor DNA using hybridization chain reaction and the pivot of triplex DNA.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lipopolysaccharides (LPS), integral components of the outer membrane of all gram-negative bacteria, are closely associated with foodborne diseases such as fever, diarrhea and hypotension, and thus, the early and sensitive detection of LPS is necessary. In this study, an aptasensor assay based on hybridization chain reaction (HCR) was developed to detect LPS. Briefly, two complementary stable species of biotinylated DNA hairpins coexisted in solution until the introduction of a detection probe triggered a hybridization chain reaction cascade. The DNA conjugates specifically reacted with the LPS, which were captured by the ethanolamine aptamer attached to the reaction well surface. After optimizing the key reaction conditions, such as the reaction time of HCR, the amount of the capture probe and detection probes, the increase in the LPS concentration was readily measured by the optical density value, and a relatively low detection limit (1.73 ng/mL) was obtained, with a linear response range of 1-10(5 )ng/mL. The approach presented herein introduced the use of an aptasensor for LPS discrimination and HCR for signal amplification, offering a promising option for detecting LPS.
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