Evidence map›Paper›PMID 27404517›Full record

ArticleJournal of visualized experiments : JoVE2016

Stem-cell Based Engineered Immunity Against HIV Infection in the Humanized Mouse Model.

Anjie Zhen, Valerie Rezek, Cindy Youn, Jonathan Rick, Brianna Lam, Nelson Chang, Jerome Zack, Masakazu Kamata, Scott Kitchen

Open access · bronzeAbstract readVideo-Audio Media
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 19 citations in OpenAlex.

  1. Article
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  8. Article
  9. Review
  10. Review
  11. Lentiviral Vector-Based Dendritic Cell Vaccine Suppresses HIV Replication in Humanized Mice.Molecular therapy : the journal of the American Society of Gene Therapy · 2019
    Article
  12. Review
  13. Review
  14. Novel AIDS therapies based on gene editing.Cellular and molecular life sciences : CMLS · 2017
    Review
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Anjie ZhenDavid Geffen School of Medicine, University of California, Los Angeles; azhen@mednet.ucla.edu.
Valerie RezekDavid Geffen School of Medicine, University of California, Los Angeles.
Cindy YounDavid Geffen School of Medicine, University of California, Los Angeles.
Jonathan RickDavid Geffen School of Medicine, University of California, Los Angeles.
Brianna LamDavid Geffen School of Medicine, University of California, Los Angeles.
Nelson ChangDavid Geffen School of Medicine, University of California, Los Angeles.
Jerome ZackDavid Geffen School of Medicine, University of California, Los Angeles.
Masakazu KamataDavid Geffen School of Medicine, University of California, Los Angeles.
Scott KitchenDavid Geffen School of Medicine, University of California, Los Angeles; skitchen@ucla.edu.
University of California, Los Angeles · US

Funding

Virology CoreP30AI028697 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ANTON, PETER A · 1991 to 2018
$39.9M
The role of CD4 in macrophage differentiation and functionR01AI078806 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI KITCHEN, SCOTT G · 2009 to 2013
$1.9M
NIAID NIH HHS P30 AI028697NIAID NIH HHS R01 AI078806
6 · The paper itself

Abstract

With the rapid development of stem cell-based gene therapies against HIV, there is pressing requirement for an animal model to study the hematopoietic differentiation and immune function of the genetically modified cells. The humanized Bone-marrow/Liver/Thymus (BLT) mouse model allows for full reconstitution of a human immune system in the periphery, which includes T cells, B cells, NK cells and monocytes. The human thymic implant also allows for thymic selection of T cells in autologous thymic tissue. In addition to the study of HIV infection, the model stands as a powerful tool to study differentiation, development and functionality of cells derived from hematopoietic stem cells (HSCs). Here we outline the construction of humanized non-obese diabetic (NOD)-severe combined immunodeficient (SCID)-common gamma chain knockout (cγ(-/-))-Bone-marrow/Liver/Thymus (NSG-BLT) mice with HSCs transduced with CD4 chimeric antigen receptor (CD4CAR) lentivirus vector. We show that the CD4CAR HSCs can successfully differentiate into multiple lineages and have anti-HIV activity. The goal of the study is to demonstrate the use of NSG-BLT mouse model as an in vivo model for engineered immunity against HIV. It is worth noting that, because lentivirus and human tissue is used, experiments and surgeries should be performed in a Class II biosafety cabinet in a Biosafety Level 2 (BSL2) with special precautions (BSL2+) facility.

Indexed as

HIV InfectionsAnimalsDisease Models, AnimalHematopoietic Stem CellsHematopoietic Stem Cell TransplantationHumansMiceMice, Inbred NODMice, SCID

Identifiers

PMID27404517
PMCPMC4993349
OpenAlexW2474664089

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.