ArticleScientific reports2016
An invertebrate-specific and immune-responsive microRNA augments oyster haemocyte phagocytosis by targeting CgIκB2.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- A microRNA Catalogue for Red Abalone Larvae Reveals Developmental and Acidification-Associated Abundance Patterns.Marine biotechnology (New York, N.Y.) · 2026Article
- Function and Development of Deep-sea Mussel Bacteriocytes Revealed by snRNA-seq and Spatial Transcriptomics.Genomics, proteomics & bioinformatics · 2026Article
- Review
- Exploring the Role of a Novel Interleukin-17 Homolog from Invertebrate Marine MusselInternational journal of molecular sciences · 2023Article
- Identification and Characterization of miRNAs and Their Predicted mRNAs in the Larval Development of Pearl Oyster Pinctada fucata.Marine biotechnology (New York, N.Y.) · 2022Article
- Soluble mediators of innate immunity in annelids and bivalve mollusks: A mini-review.Frontiers in immunology · 2022Review
- Discovery and functional understanding of MiRNAs in molluscs: a genome-wide profiling approach.RNA biology · 2021Article
- Digging into bivalve miRNAomes: between conservation and innovation.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Review
- Genetic features of Haliotis discus hannai by infection of vibrio and virus.Genes & genomics · 2020Review
- MicroRNAs in Daphnia magna identified and characterized by deep sequencing, genome mapping and manual curation.Scientific reports · 2019Article
- An invertebrate-specific miRNA targeted the ancient cholinergic neuroendocrine system of oyster.Open biology · 2016Article
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear factor (NF)-κB pathway is an evolutionally conserved pathway in activating immune response, in which IκBs can repress the activation. In the present study, cgi-miR-2d, an invertebrate-specific microRNA, was proved to regulate CgIκB2 expression and haemocyte phagocytosis during bacterial infection in oyster Crassostrea gigas. The expression of cgi-miR-2d was significantly up-regulated after Vibrio splendidus challenge, while CgIκB2 transcripts decreased. Significant decreases in both luminescence and CgIκB2 3'UTR level was observed after transfection of cgi-miR-2d in CgIκB2 3'UTR luciferase reporter assay. CgIκB2 mRNA level decreased significantly (0.51-fold of control group, p < 0.05) in gain-of-function assay of cgi-miR-2d in vivo while it increased markedly (1.27-fold, p < 0.05) when cgi-miR-2d was repressed (0.10-fold, p < 0.01). A significant increase of haemocyte phagocytosis rate was observed in cgi-miR-2d overexpression group (p < 0.01), consistent with results in CgIκB2 knock-down group (p < 0.01). Moreover, the apoptosis rate of haemocytes was found significantly declined (28.57%, p < 0.01) in gain-of-function assay of cgi-miR-2d. Together, those results not only depicted the functional conservation of miR-2d family in anti-apoptosis of oysters but also highlighted its interaction with phagocytosis by modulating NF-κB pathway, which might dedicate critically to the well-balance of host immune response.
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