ArticleScientific reports2016
Analyzing the CDR3 Repertoire with respect to TCR-Beta Chain V-D-J and V-J Rearrangements in Peripheral T Cells using HTS.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Identifying expanding TCR clonotypes with a longitudinal Bayesian mixture model and their associations with cancer patient prognosis, metastasis-directed therapy, and VJ gene enrichment.Bioinformatics (Oxford, England) · 2026Article
- Chain-specific remodeling of the γδ T-cell TCR repertoire in preterm infants with cCMV: a gestational-age-stratified case-control study.Frontiers in immunology · 2026Article
- A High-Throughput Sequencing Strategy for Clinical Repertoire Profiling of T Cell Receptor Beta Chain: Development and Reference Values Across Healthy Adults, Paediatrics, and Cord Blood Units.International journal of molecular sciences · 2025Article
- scTCR-seq and HTS reveal a special novel TRBD2-TRBJ1 rearrangement in mammalian TRB CDR3 repertoire.BMC genomics · 2025Article
- The T-cell receptor β chain CDR3 insights of bovine liver immune repertoire under heat stress.Animal bioscience · 2024Article
- HTS and scRNA-seq revealed that the location and RSS quality of the mammalian TRBV and TRBJ genes impact biased rearrangement.BMC genomics · 2024Article
- Article
- The reverse TRBV30 gene of mammals: a defect or superiority in evolution?BMC genomics · 2024Article
- TCR repertoire landscape reveals macrophage-mediated clone deletion in endotoxin tolerance.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2023Article
- Two Step Selection for Bias inFrontiers in immunology · 2022Article
- TCRβ rearrangements without a D segment are common, abundant, and public.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Review
- V(DD)J recombination is an important and evolutionarily conserved mechanism for generating antibodies with unusually long CDR3s.Genome research · 2020Article
- Single-cell TCR sequencing reveals phenotypically diverse clonally expanded cells harboring inducible HIV proviruses during ART.Nature communications · 2020Article
- Revealing factors determining immunodominant responses against dominant epitopes.Immunogenetics · 2020Review
- Analysis of the Heterogeneity of CD4Journal of immunology research · 2020Article
- Replacement of TCR Dβ With Immunoglobulin DFrontiers in immunology · 2020Article
- Heterogeneity of CD4Frontiers in cell and developmental biology · 2020Article
- Preliminary analysis of spatial-temporal homogeneity and heterogeneity of TCR β chain CDR3 repertoires in BALB/c mice.International journal of molecular epidemiology and genetics · 2019Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
V-D-J rearrangement of the TCR-beta chain follows the 12/23 rule and the beyond 12/23 restriction. Currently, the proportion and characteristics of TCR-beta chain V-J rearrangement is unclear. We used high-throughput sequencing to compare and analyze TCR-beta chain V-J rearrangement and V-D-J rearrangement in the CDR3 repertoires of T cells from the PBMCs of six volunteers and six BALB/c mice. The results showed that the percentage of V-J rearrangement of the volunteers was approximately 0.7%, whereas that of the mice was 2.2%. The clonality of mice V-J rearrangement was significantly reduced compared with the V-D-J rearrangement, whereas the clonality of human V-J rearrangement was slightly reduced compared with the V-D-J rearrangement. V-J rearrangement in CDR3 involved the significant usage of N, S, F and L, whereas V-D-J rearrangement in CDR3 involved the significant usage of R and G. The levels of V deletion and J deletion in V-J rearrangement were significantly reduced compared with V-D-J rearrangement. TRBD and TRBJ usage in V-J rearrangement differed from that of V-D-J rearrangement, including dominant usage of TRBV and TRBJ and their pairing. Taken together, these results provide new ideas and technology for studies of V-D-J rearrangement and V-J rearrangement in the CDR3 repertoire.
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