ArticleScientific reports2016
Endothelial cell alignment as a result of anisotropic strain and flow induced shear stress combinations.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Angiogenic Potential of Endothelial Cells in Response to the Stiffness and Anisotropy of Right Ventricle Mimetic Scaffolds.Bioengineering (Basel, Switzerland) · 2026Article
- Investigational New Drug-enabling studies in a human vessel-chip: Are we there yet?Bioengineering & translational medicine · 2026Review
- Vascular endothelial integration of multiple biophysical stimuli.Frontiers in cardiovascular medicine · 2026Article
- Design and performance analysis of bionic trabecular bone scaffold structure based on Voronoi.Journal of biological engineering · 2025Article
- Uniaxial cyclic stretch regulates the expression of thrombomodulin and von Willebrand factor on endothelial cells.Blood vessels, thrombosis & hemostasis · 2025Article
- Temporal dynamics of angiogenesis: the emerging role of mechanoregulated pathways.Biochemical Society transactions · 2025Review
- Leveraging femtosecond laser machining for the fabrication of tubular-based Organ-on-Chip systems: modeling cancer metastasis from invasion to intravasation.Materials today. Bio · 2025Article
- Endothelial Dysfunction in Atherosclerosis: Experimental Models and Therapeutics.Biomaterials research · 2025Review
- Under pressure: integrated endothelial cell response to hydrostatic and shear stresses.Vascular biology (Bristol, England) · 2025Article
- Helical vasculogenesis driven by cell chirality.Science advances · 2024Article
- NanoIEA: A Nanopatterned Interdigitated Electrode Array-Based Impedance Assay for Real-Time Measurement of Aligned Endothelial Cell Barrier Functions.Advanced healthcare materials · 2024Article
- Analysis of flow-induced transcriptional response and cell alignment of different sources of endothelial cells used in vascular tissue engineering.Scientific reports · 2023Article
- Engineered Human Tissue as A New Platform for Mosquito Bite-Site Biology Investigations.Insects · 2023Article
- Design and 3D Printing of Personalized Hybrid and Gradient Structures for Critical Size Bone Defects.ACS applied bio materials · 2023Article
- The role of the blood-brain barrier during neurological disease and infection.Biochemical Society transactions · 2023Review
- Polymeric and biological membranes for organ-on-a-chip devices.Microsystems & nanoengineering · 2023Review
- A 96-wells fluidic system for high-throughput screenings under laminar high wall shear stress conditions.Microsystems & nanoengineering · 2023Article
- Effect of mechanical forces on cellular response to radiation.Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology · 2022Review
- Article
- Joint-on-chip platforms: entering a new era of in vitro models for arthritis.Nature reviews. Rheumatology · 2022Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endothelial cells (ECs) are continuously exposed in vivo to cyclic strain and shear stress from pulsatile blood flow. When these stimuli are applied in vitro, ECs adopt an appearance resembling their in vivo state, most apparent in their alignment (perpendicular to uniaxial strain and along the flow). Uniaxial strain and flow perpendicular to the strain, used in most in vitro studies, only represent the in vivo conditions in straight parts of vessels. The conditions present over large fractions of the vasculature can be better represented by anisotropic biaxial strains at various orientations to flow. To emulate these biological complexities in vitro, we have developed a medium-throughput device to screen for the effects on cells of variously oriented anisotropic biaxial strains and flow combinations. Upon the application of only strains for 24 h, ECs (HUVECs) aligned perpendicular to the maximum principal strain and the alignment was stronger for a higher maximum:minimum principal strain ratio. A 0.55 Pa shear stress, when applied alone or with strain for 24 h, caused cells to align along the flow. Studying EC response to such combined physiological mechanical stimuli was not possible with existing platforms and to our best knowledge, has not been reported before.
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