ArticleScientific reports2016
Heritable genome editing with CRISPR/Cas9 induces anosmia in a crop pest moth.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 183 citations in OpenAlex.
- Harnessing next-generation molecular technologies for precision control of Tuta absoluta and Helicoverpa armigera in tomato.Functional & integrative genomics · 2026Review
- Basic Research on Whitefly Molecular Biology: A Foundation for Innovative Pest Management Strategies.Current issues in molecular biology · 2026Review
- Molecular bases of insect odorant receptor function: specificity and evolution.Biological reviews of the Cambridge Philosophical Society · 2026Review
- Neoclassical development of genetic sexing strains for insect pest and disease vector control.Insect science · 2026Review
- Emerging tools to advance neuroethology in butterflies and moths.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2026Review
- Identification of two odorant receptors tuned to alarm pheromone compounds in the honey bee Apis mellifera.Communications biology · 2025Article
- Managing African Armyworm Outbreaks in Sub-Saharan Africa: Current Strategies and Future Directions.Insects · 2025Review
- Where do all the pests go? Understanding the genomic mechanisms of crop pest dynamics during the off-season.Current opinion in insect science · 2025Review
- Manipulation of a New Non-model Insect Genome Using Targeted CRISPR-Era Approaches.Methods in molecular biology (Clifton, N.J.) · 2025Article
- CRISPR/Cas9-Based Genome Editing of Fall Armyworm (Biomolecules · 2024Review
- CRISPR/Cas9-mediated genome editing technique to control fall armyworm (Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
- Odorant receptor co-receptors affect expression of tuning receptors inFrontiers in cellular neuroscience · 2024Article
- Odorant receptors for floral- and plant-derived volatiles in the yellow fever mosquito, Aedes aegypti (Diptera: Culicidae).PloS one · 2024Article
- Identification of candidate chemosensory genes inFrontiers in physiology · 2024Article
- CRISPR/Cas9: a cutting-edge solution for combatting the fall armyworm, Spodoptera frugiperda.Molecular biology reports · 2023Review
- Biotechnology and Solutions: Insect-Pest-Resistance Management for Improvement and Development of Bt Cotton (Plants (Basel, Switzerland) · 2023Article
- Unveiling the Genetic Symphony: Harnessing CRISPR-Cas Genome Editing for Effective Insect Pest Management.Plants (Basel, Switzerland) · 2023Review
- Enhancement of Pathogen Toxicity by FeedingInsects · 2023Article
- Multisite imaging of neural activity using a genetically encoded calcium sensor in the honey bee.PLoS biology · 2023Article
- Advancements and prospects of CRISPR/Cas9 technologies for abiotic and biotic stresses in sugar beet.Frontiers in genetics · 2023Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lepidoptera suffer critical lack of genetic tools and heritable genome edition has been achieved only in a few model species. Here we demonstrate that the CRISPR/Cas9 system is highly efficient for genome editing in a non-model crop pest Lepidoptera, the noctuid moth Spodoptera littoralis. We knocked-out the olfactory receptor co-receptor Orco gene to investigate its function in Lepidoptera olfaction. We find that 89.6% of the injected individuals carried Orco mutations, 70% of which transmitted them to the next generation. CRISPR/Cas9-mediated Orco knockout caused defects in plant odor and sex pheromone olfactory detection in homozygous individuals. Our work genetically defines Orco as an essential OR partner for both host and mate detection in Lepidoptera, and demonstrates that CRISPR/Cas9 is a simple and highly efficient genome editing technique in noctuid pests opening new routes for gene function analysis and the development of novel pest control strategies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.