ArticleScientific reports2016
A metabolic intermediate of the fructose-asparagine utilization pathway inhibits growth of a Salmonella fraB mutant.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 32 citations in OpenAlex.
- Metabolic strategies of Enterobacteriaceae gut colonization.Nature reviews. Microbiology · 2026Review
- The Gfr Uptake System Provides a Context-Dependent Fitness Advantage to Salmonella Typhimurium SL1344 During the Initial Gut Colonization Phase.Molecular microbiology · 2025Article
- Development of Functional Msalais Wines Rich in Amadori Compounds by Yeast Fermentation.Foods (Basel, Switzerland) · 2025Article
- Crystal structures of Salmonella enterica FraB deglycase reveal a conformational heterodimer with remarkable structural plasticity at the active site.Protein science : a publication of the Protein Society · 2025Article
- Identification of inhibitors of the Salmonella FraB deglycase, a drug target.FEBS open bio · 2025Article
- MtlD as a therapeutic target for intestinal and systemic bacterial infections.Journal of bacteriology · 2025Article
- Polarity effects, resistance, and probiotic enhancement of intoxication of Salmonella enterica fraB mutants in murine models.PloS one · 2025Article
- Lethal metabolism of Candida albicans respiratory mutants.PloS one · 2024Article
- Insights into the catalytic mechanism of a bacterial deglycase essential for utilization of fructose-lysine.Protein science : a publication of the Protein Society · 2023Article
- Demonstrating the utility of sugar-phosphate phosphatases in coupled enzyme assays: galactose-1-phosphate uridylyltransferase as proof-of-concept.Glycobiology · 2023Article
- Sugar-Phosphate Toxicities Attenuate Salmonella Fitness in the Gut.Journal of bacteriology · 2022Article
- Inducing Self-Poisoning: a Feasible Antibacterial Strategy?Journal of bacteriology · 2022Article
- Serendipitous Discovery of a Competitive Inhibitor of FraB, aPathogens (Basel, Switzerland) · 2022Article
- Characterization of a Salmonella Transcription Factor-DNA Complex and Identification of the Inducer by Native Mass Spectrometry.Journal of molecular biology · 2022Article
- Effect of diet on pathogen performance in the microbiome.Microbiome research reports · 2022Review
- Integrated Use of Biochemical, Native Mass Spectrometry, Computational, and Genome-Editing Methods to Elucidate the Mechanism of a Salmonella deglycase.Journal of molecular biology · 2019Article
- Salmonella-Mediated Inflammation Eliminates Competitors for Fructose-Asparagine in the Gut.Infection and immunity · 2018Article
- Identification of Bacterial Species That Can Utilize Fructose-Asparagine.Applied and environmental microbiology · 2018Article
- Measurement of Fructose-Asparagine Concentrations in Human and Animal Foods.Journal of agricultural and food chemistry · 2018Article
- Salmonella FraE, an Asparaginase Homolog, Contributes to Fructose-Asparagine but Not Asparagine Utilization.Journal of bacteriology · 2017Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
Abstract
Insertions in the Salmonella enterica fra locus, which encodes the fructose-asparagine (F-Asn) utilization pathway, are highly attenuated in mouse models of inflammation (>1000-fold competitive index). Here, we report that F-Asn is bacteriostatic to a fraB mutant (IC50 19 μM), but not to the wild-type or a fra island deletion mutant. We hypothesized that the presence of FraD kinase and absence of FraB deglycase causes build-up of a toxic metabolite: 6-phosphofructose-aspartate (6-P-F-Asp). We used biochemical assays to assess FraB and FraD activities, and mass spectrometry to confirm that the fraB mutant accumulates 6-P-F-Asp. These results, together with our finding that mutants lacking fraD or the fra island are not attenuated in mice, suggest that the extreme attenuation of a fraB mutant stems from 6-P-F-Asp toxicity. Salmonella FraB is therefore an excellent drug target, a prospect strengthened by the absence of the fra locus in most of the gut microbiota.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.