ArticleJournal of visualized experiments : JoVE2016
Removal of Exogenous Materials from the Outer Portion of Frozen Cores to Investigate the Ancient Biological Communities Harbored Inside.
Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
6 citing papers in PubMed.
- High-quality draft genome sequence ofMicrobiology resource announcements · 2026Article
- Active layer and permafrost microbial community coalescence increases soil activity and diversity in mixed communities compared to permafrost alone.Frontiers in microbiology · 2025Article
- A Case Study for the Recovery of Authentic Microbial Ancient DNA from Soil Samples.Microorganisms · 2022Article
- The role of changing temperature in microbial metabolic processes during permafrost thaw.PloS one · 2020Article
- Meeting report of the third annual Tri-Service Microbiome Consortium symposium.Environmental microbiome · 2020Article
- Potential microbial contamination during sampling of permafrost soil assessed by tracers.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cryosphere offers access to preserved organisms that persisted under past environmental conditions. In fact, these frozen materials could reflect conditions over vast time periods and investigation of biological materials harbored inside could provide insight of ancient environments. To appropriately analyze these ecosystems and extract meaningful biological information from frozen soils and ice, proper collection and processing of the frozen samples is necessary. This is especially critical for microbial and DNA analyses since the communities present may be so uniquely different from modern ones. Here, a protocol is presented to successfully collect and decontaminate frozen cores. Both the absence of the colonies used to dope the outer surface and exogenous DNA suggest that we successfully decontaminated the frozen cores and that the microorganisms detected were from the material, rather than contamination from drilling or processing the cores.
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Registered trials
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