ArticleProceedings of the National Academy of Sciences of the United States of America1989
F-actin affinity chromatography: technique for isolating previously unidentified actin-binding proteins.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- The Molecular Aspects of Functional Activity of Macrophage-Activating Factor GcMAF.International journal of molecular sciences · 2023Article
- Functional comparison of full-length palladin to isolated actin binding domain.Protein science : a publication of the Protein Society · 2023Article
- A Comprehensive Guide for Performing Sample Preparation and Top-Down Protein Analysis.Proteomes · 2017Review
- Anillin regulates cell-cell junction integrity by organizing junctional accumulation of Rho-GTP and actomyosin.Current biology : CB · 2014Article
- AtPID: Arabidopsis thaliana protein interactome database--an integrative platform for plant systems biology.Nucleic acids research · 2008Article
- Interactions among a fimbrin, a capping protein, and an actin-depolymerizing factor in organization of the fission yeast actin cytoskeleton.Molecular biology of the cell · 2001Article
- Toxofilin, a novel actin-binding protein from Toxoplasma gondii, sequesters actin monomers and caps actin filaments.Molecular biology of the cell · 2000Article
- 2E4 (kaptin): a novel actin-associated protein from human blood platelets found in lamellipodia and the tips of the stereocilia of the inner ear.European journal of cell biology · 1999Article
- Isolation of actin-associated proteins from Caenorhabditis elegans oocytes and their localization in the early embryo.The EMBO journal · 1997Article
- Tubulin domains for the interaction of microtubule associated protein DMAP-85 from Drosophila melanogaster.Molecular and cellular biochemistry · 1996Article
- A talin homologue of Dictyostelium rapidly assembles at the leading edge of cells in response to chemoattractant.The Journal of cell biology · 1995Article
- Protein-protein interactions: methods for detection and analysis.Microbiological reviews · 1995Review
- Cytoskeletal domains in the activated platelet.Cell motility and the cytoskeleton · 1995Article
- Identification of secreted and cytosolic gelsolin in Drosophila.The Journal of cell biology · 1994Article
- Actin structure and function: roles in mitochondrial organization and morphogenesis in budding yeast and identification of the phalloidin-binding site.Molecular biology of the cell · 1993Article
- Maternal effect mutations of the sponge locus affect actin cytoskeletal rearrangements in Drosophila melanogaster embryos.The Journal of cell biology · 1992Article
- An unconventional myosin heavy chain gene from Drosophila melanogaster.The Journal of cell biology · 1992Article
- Purification of a multiprotein complex containing centrosomal proteins from the Drosophila embryo by chromatography with low-affinity polyclonal antibodies.Molecular biology of the cell · 1992Article
- Sequential rearrangement and nuclear polymerization of actin in baculovirus-infected Spodoptera frugiperda cells.Journal of virology · 1991Article
- Actin-binding proteins from Drosophila embryos: a complex network of interacting proteins detected by F-actin affinity chromatography.The Journal of cell biology · 1989Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We have developed stable and easy to use filamentous actin (F-actin) affinity-chromatography columns that selectively purify proteins that bind to actin filaments from cell extracts. Most traditional assays for actin-associated proteins screen for their effects on actin polymerization or actin filament crosslinking. Because our technique requires only actin-filament binding, it can identify additional types of proteins involved in the function of the actin cytoskeleton. By chromatographing extracts of several types of cells on these columns, we show that known actin-binding proteins are selectively retained as a subset of a larger group of actin-binding proteins that have not been identified previously.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.