Evidence map›Paper›PMID 27403167›Full record

ArticleStem cells international2016

Cryopreservation of Bone Marrow Mononuclear Cells Alters Their Viability and Subpopulation Composition but Not Their Treatment Effects in a Rodent Stroke Model.

Bing Yang, Kaushik Parsha, Krystal Schaar, Nikunj Satani, Xiaopei Xi, Jaroslaw Aronowski, Sean I Savitz

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells international, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Article
  6. Article
  7. Cryopreservation of Hematopoietic Stem Cells: Emerging Assays, Cryoprotectant Agents, and Technology to Improve Outcomes.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2019
    Review
  8. Technical Considerations in the Freezing, Low-Temperature Storage and Thawing of Stem Cells for Cellular Therapies.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2019
    Review
  9. Article
  10. Best practices for cryopreserving, thawing, recovering, and assessing cells.In vitro cellular & developmental biology. Animal · 2017
    Review
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Bing YangStroke Program, Department of Neurology, McGovern Medical School, University of Texas Health Science Center, Houston, TX 77030, USA.ORCID 0000-0001-6860-583X
Kaushik ParshaStroke Program, Department of Neurology, McGovern Medical School, University of Texas Health Science Center, Houston, TX 77030, USA.
Krystal SchaarStroke Program, Department of Neurology, McGovern Medical School, University of Texas Health Science Center, Houston, TX 77030, USA.
Nikunj SataniStroke Program, Department of Neurology, McGovern Medical School, University of Texas Health Science Center, Houston, TX 77030, USA.ORCID 0000-0003-3908-3731
Xiaopei XiStroke Program, Department of Neurology, McGovern Medical School, University of Texas Health Science Center, Houston, TX 77030, USA.
Jaroslaw AronowskiStroke Program, Department of Neurology, McGovern Medical School, University of Texas Health Science Center, Houston, TX 77030, USA.
Sean I SavitzStroke Program, Department of Neurology, McGovern Medical School, University of Texas Health Science Center, Houston, TX 77030, USA.
The University of Texas Health Science Center at Houston · US

Funding

Cytoprotection with Autologous Mononuclear Cells for StrokeR01NS071127 · NINDS · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI SAVITZ, SEAN I · 2011 to 2015
$1.6M
NINDS NIH HHS R01 NS071127
6 · The paper itself

Abstract

The systemic administration of autologous bone marrow (BM) derived mononuclear cells (MNCs) is under investigation as a novel therapeutic modality for the treatment of ischemic stroke. Autologous applications raise the possibility that MNCs could potentially be stored as a banked source. There have been no studies that investigate the effects of cryopreservation of BM-MNCs on their functional abilities in stroke models. In the present study, C57BL/6 mice were subjected to middle cerebral artery occlusion (MCAo) for 60 minutes and then divided into two treatment groups: fresh MNCs versus cryopreserved MNCs. BM-MNCs were collected at 22 hours after MCAo and were stored in liquid nitrogen for 12 months in cryopreserved MNCs group. BM-MNCs cellular viability, composition, and phenotype of the various subpopulations of mice BM-MNCs were evaluated by flow cytometry, and the behavioral recovery of stroke animals was tested with freshly harvested MNCs versus cryopreserved MNCs by corner test and ladder rung test. We found that long-term cryopreservation negatively impacts the cellular viability of bone marrow MNCs. Cryopreservation also alters the cellular composition of various subpopulations within the MNCs. However, despite the changes observed in cryopreserved cells, both fresh and frozen MNCs have similar beneficial effect on behavioral and histological outcomes.

Identifiers

PMID27403167
PMCPMC4926012
OpenAlexW2424499627

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.