ArticleBiology open2016
Focal adhesion kinase is required for actin polymerization and remodeling of the cytoskeleton during sperm capacitation.
Article in Biology open, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 51 citations in OpenAlex.
- Alterations of actin in aging.Journal of cell science · 2026Review
- Large cyclic AMP-dependent increases in tyrosine-phosphorylated proteins are not absolutely necessary for the induction of full-type hyperactivation and penetration into eggs in ejaculated boar spermatozoa in vitro.The Journal of reproduction and development · 2026Article
- Article
- TMEM16A Maintains Acrosomal Integrity Through ERK1/2, RhoA, and Actin Cytoskeleton During Capacitation.International journal of molecular sciences · 2025Article
- Paxillin and Kindlin: Research Progress and Biological Functions.Biomolecules · 2025Review
- Evolutionarily conserved ovarian fluid proteins are responsible for extending egg viability in salmonid fish.Scientific reports · 2024Article
- Testis-specific actin-like 7A (ACTL7A) is an indispensable protein for subacrosomal-associated F-actin formation, acrosomal anchoring, and male fertility.Molecular human reproduction · 2023Article
- Calpain Regulates Reactive Oxygen Species Production during Capacitation through the Activation of NOX2 and NOX4.International journal of molecular sciences · 2023Article
- Role of cytoskeleton-related proteins in the acrosome reaction of Eriocheir sinensis spermatozoa.BMC genomic data · 2023Article
- Effects of phytoestrogens combined with cold stress on sperm parameters and testicular proteomics in rats.Open life sciences · 2023Article
- Prepubertal nutritional modulation in the bull and its impact on sperm DNA methylation.Cell and tissue research · 2022Article
- Candidate genes for infertility: an in-silico study based on cytogenetic analysis.BMC medical genomics · 2022Article
- The cryopreservation process induces alterations in proteins associated with bull sperm quality: The equilibration process could be a probable critical control point.Frontiers in endocrinology · 2022Article
- Article
- Pyk2 regulates cell-edge protrusion dynamics by interacting with Crk.Molecular biology of the cell · 2021Article
- Article
- Seminal Plasma: Relevant for Fertility?International journal of molecular sciences · 2021Review
- Identification of the RecombinantFrontiers in cell and developmental biology · 2021Article
- Ovarian fluid proteome variation associates with sperm swimming speed in an externally fertilizing fish.Journal of evolutionary biology · 2020Article
- ACTN4 Mediates SEPT14 Mutation-Induced Sperm Head Defects.Biomedicines · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several focal adhesion proteins are known to cooperate with integrins to link the extracellular matrix to the actin cytoskeleton; as a result, many intracellular signaling pathways are activated and several focal adhesion complexes are formed. However, how these proteins function in mammalian spermatozoa remains unknown. We confirm the presence of focal adhesion proteins in guinea pig spermatozoa, and we explore their role during capacitation and the acrosome reaction, and their relationship with the actin cytoskeleton. Our results suggest the presence of a focal adhesion complex formed by β1-integrin, focal adhesion kinase (FAK), paxillin, vinculin, talin, and α-actinin in the acrosomal region. Inhibition of FAK during capacitation affected the protein tyrosine phosphorylation associated with capacitation that occurs within the first few minutes of capacitation, which caused the acrosome reaction to become increasingly Ca(2+) dependent and inhibited the polymerization of actin. The integration of vinculin and talin into the complex, and the activation of FAK and paxillin during capacitation, suggests that the complex assembles at this time. We identify that vinculin and α-actinin increase their interaction with F-actin while it remodels during capacitation, and that during capacitation focal adhesion complexes are structured. FAK contributes to acrosome integrity, likely by regulating the polymerization and the remodeling of the actin cytoskeleton.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.