ArticlePlanta2016
Combination of transcriptomic and proteomic approaches helps to unravel the protein composition of Chelidonium majus L. milky sap.
Article in Planta, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
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- Effects of topping and non-topping on growth-regulating hormones of flue-cured tobacco (Frontiers in plant science · 2023Article
- The Activity ofInternational journal of molecular sciences · 2022Article
- Tap the sap - investigation of latex-bearing plants in the search of potential anticancer biopharmaceuticals.Frontiers in plant science · 2022Review
- Functional Studies of Plant Latex as a Rich Source of Bioactive Compounds: Focus on Proteins and Alkaloids.International journal of molecular sciences · 2021Review
- Combined Protein and Alkaloid Research ofInternational journal of molecular sciences · 2021Article
- Advances in studies on ion transporters involved in salt tolerance and breeding crop cultivars with high salt tolerance.Journal of Zhejiang University. Science. B · 2020Review
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- Proteomic approach to understand the molecular physiology of symbiotic interaction between Piriformospora indica and Brassica napus.Scientific reports · 2018Article
- Greater Celandine's Ups and Downs-21 Centuries of Medicinal Uses ofFrontiers in pharmacology · 2018Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MAIN
conclusionA novel annotated Chelidonium majus L. transcriptome database composed of 23,004 unique coding sequences allowed to significantly improve the sensitivity of proteomic C. majus assessments, which showed novel defense-related proteins characteristic to its latex. To date, the composition of Chelidonium majus L. milky sap and biosynthesis of its components are poorly characterized. We, therefore, performed de novo sequencing and assembly of C. majus transcriptome using Illumina technology. Approximately, 119 Mb of raw sequence data was obtained. Assembly resulted in 107,088 contigs, with N50 of 1913 bp and N90 of 450 bp. Among 34,965 unique coding sequences (CDS), 23,004 obtained CDS database served as a basis for further proteomic analyses. The database was then used for the identification of proteins from C. majus milky sap, and whole plant extracts analyzed using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) approach. Of about 334 different putative proteins were identified in C. majus milky sap and 1155 in C. majus whole plant extract. The quantitative comparative analysis confirmed that C. majus latex contains proteins connected with response to stress conditions and generation of precursor metabolites and energy. Notable proteins characteristic to latex include major latex protein (MLP, presumably belonging to Bet v1-like superfamily), polyphenol oxidase (PPO, which could be responsible for browning of the sap after exposure to air), and enzymes responsible for anthocyanidin, phenylpropanoid, and alkaloid biosynthesis.
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