Evidence map›Paper›PMID 27400714›Full record

ArticleThe American journal of sports medicine2016

Platelet-Rich Plasma Activates Proinflammatory Signaling Pathways and Induces Oxidative Stress in Tendon Fibroblasts.

Joshua L Hudgens, Kristoffer B Sugg, Jeremy A Grekin, Jonathan P Gumucio, Asheesh Bedi, Christopher L Mendias

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In one paragraph

Article in The American journal of sports medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
79citing papers in PubMed, 3 pooled it
11.3field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

79 citing papers in PubMed, 3 syntheses or guidelines pooled it, 136 citations in OpenAlex.

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19 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Joshua L HudgensDepartment of Orthopaedic Surgery, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Kristoffer B SuggDepartment of Orthopaedic Surgery, University of Michigan Medical School, Ann Arbor, Michigan, USA Department of Molecular & Integrative Physiology, University of Michigan Medical School, Ann Arbor, Michigan, USA Section of Plastic Surgery, Department of Surgery, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Jeremy A GrekinDepartment of Orthopaedic Surgery, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Jonathan P GumucioDepartment of Orthopaedic Surgery, University of Michigan Medical School, Ann Arbor, Michigan, USA Department of Molecular & Integrative Physiology, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Asheesh BediDepartment of Orthopaedic Surgery, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Christopher L MendiasDepartment of Orthopaedic Surgery, University of Michigan Medical School, Ann Arbor, Michigan, USA Department of Molecular & Integrative Physiology, University of Michigan Medical School, Ann Arbor, Michigan, USA cmendias@umich.edu.
University of Michigan–Ann Arbor · US

Funding

Sex Differences in Tendon Growth and RemodelingR01AR063649 · NIAMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI RODEO, SCOTT A · 2013 to 2022
$3.6M
The Role of Matrix Metalloproteinases in Tendon GrowthF32AR067086 · NIAMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI SUGG, KRISTOFFER BRADLEY · 2014 to 2016
$202k
Fatty Macrophage Regulation of Atrophy and Inflammation in Rotator Cuff TearsF31AR065931 · NIAMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI GUMUCIO, JONATHAN PAUL · 2014 to 2016
$103k
NIAMS NIH HHS F31 AR065931NIAMS NIH HHS F32 AR067086NIAMS NIH HHS R01 AR063649
6 · The paper itself

Abstract

backgroundTendon injuries are one of the most common musculoskeletal conditions in active patients. Platelet-rich plasma (PRP) has shown some promise in the treatment of tendon disorders, but little is known as to the mechanisms by which PRP can improve tendon regeneration. PRP contains numerous different growth factors and cytokines that activate various cellular signaling cascades, but it has been difficult to determine precisely which signaling pathways and cellular responses are activated after PRP treatment. Additionally, macrophages play an important role in modulating tendon regeneration, but the influence of PRP on determining whether macrophages assume a proinflammatory or anti-inflammatory phenotype remains unknown. PURPOSE: To use genome-wide expression profiling, bioinformatics, and protein analysis to determine the cellular pathways activated in fibroblasts treated with PRP. The effect of PRP on macrophage polarization was also evaluated. STUDY

designControlled laboratory study.

methodsTendon fibroblasts or macrophages from rats were cultured and treated with either platelet-poor plasma (PPP) or PRP. RNA or protein was isolated from cells and analyzed using microarrays, quantitative polymerase chain reaction, immunoblotting, or bioinformatics techniques.

resultsPathway analysis determined that the most highly induced signaling pathways in PRP-treated tendon fibroblasts were TNFα and NFκB pathways. PRP also downregulated the expression of extracellular matrix genes and induced the expression of autophagy-related genes and reactive oxygen species (ROS) genes and protein markers in tendon fibroblasts. PRP failed to have a major effect on markers of macrophage polarization.

conclusionPRP induces an inflammatory response in tendon fibroblasts, which leads to the formation of ROS and the activation of oxidative stress pathways. PRP does not appear to significantly modulate macrophage polarization. CLINICAL RELEVANCE: PRP might act by inducing a transient inflammatory event, which could then trigger a tissue regeneration response.

Indexed as

Oxidative StressAnimalsCytokinesFibroblastsHumansMacrophagesMalePlatelet-Rich PlasmaRatsRats, Inbred LewRegenerationTendon InjuriesTendonsWound HealingCytokinesautophagyinflammationoxidative stressplatelet-rich plasmatendinopathytendon

Identifiers

PMID27400714
PMCPMC4970921
OpenAlexW2463845948

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.