ArticleNature methods2016
Reversible cryo-arrest for imaging molecules in living cells at high spatial resolution.
Article in Nature methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- Robust thermometry-imaging down to sub-micrometer or millisecond-resolution by fluorescence lifetime microscopy allows for additional acquisition of multiple imaging channels.Scientific reports · 2026Article
- Time-deterministic cryo-optical microscopy.Light, science & applications · 2025Article
- Cryogenic single-molecule fluorescence imaging.BMB reports · 2025Review
- The EGFR phosphatase RPTPγ is a redox-regulated suppressor of promigratory signaling.The EMBO journal · 2023Article
- Ultrarapid cryo-arrest of living cells on a microscope enables multiscale imaging of out-of-equilibrium molecular patterns.Science advances · 2021Article
- Crosstalk in transition: the translocation of Akt.Journal of mathematical biology · 2019Article
- Using an RNA aptamer probe for super-resolution imaging of native EGFR.Nanoscale advances · 2019Article
- Quantification of protein mobility and associated reshuffling of cytoplasm during chemical fixation.Scientific reports · 2018Article
- Interdependence between EGFR and Phosphatases Spatially Established by Vesicular Dynamics Generates a Growth Factor Sensing and Responding Network.Cell systems · 2018Article
- A conformational sensor based on genetic code expansion reveals an autocatalytic component in EGFR activation.Nature communications · 2018Article
- Live-cell measurements of kinase activity in single cells using translocation reporters.Nature protocols · 2018Article
- Super-resolution optical microscopy for studying membrane structure and dynamics.Journal of physics. Condensed matter : an Institute of Physics journal · 2017Review
- Reversible Cryo-arrests of Living Cells to Pause Molecular Movements for High-resolution Imaging.Bio-protocol · 2017Article
- Let There Be Light!Proteomes · 2016Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
The dynamics of molecules in living cells hampers precise imaging of molecular patterns by functional and super-resolution microscopy. We developed a method that circumvents lethal chemical fixation and allows on-stage cryo-arrest for consecutive imaging of molecular patterns within the same living, but arrested, cells. The reversibility of consecutive cryo-arrests was demonstrated by the high survival rate of different cell lines and by intact growth factor signaling that was not perturbed by stress response. Reversible cryo-arrest was applied to study the evolution of ligand-induced receptor tyrosine kinase activation at different scales. The nanoscale clustering of epidermal growth factor receptor (EGFR) in the plasma membrane was assessed by single-molecule localization microscopy, and endosomal microscale activity patterns of ephrin receptor A2 (EphA2) were assessed by fluorescence lifetime imaging microscopy. Reversible cryo-arrest allows the precise determination of molecular patterns while conserving the dynamic capabilities of living cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.