Evidence map›Paper›PMID 27398974›Full record

ArticleThe American journal of pathology2016

Hyaluronan Depolymerization by Megakaryocyte Hyaluronidase-2 Is Required for Thrombopoiesis.

Aaron C Petrey, Dana R Obery, Sean P Kessler, Bruno Flamion, Carol A de la Motte

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The American journal of pathology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
1.9field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 28 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Hyaluronan: An Architect and Integrator for Cancer and Neural Diseases.International journal of molecular sciences · 2025
    Review
  5. TSG6 hyaluronan matrix remodeling dampens the inflammatory response during colitis.Matrix biology : journal of the International Society for Matrix Biology · 2023
    Article
  6. Review
  7. Article
  8. Review
  9. Review
  10. Article
  11. Article
  12. New Insights Into the Differentiation of Megakaryocytes From Hematopoietic Progenitors.Arteriosclerosis, thrombosis, and vascular biology · 2019
    Review
  13. Hyaluronan in inflammatory bowel disease: Cross-linking inflammation and coagulation.Matrix biology : journal of the International Society for Matrix Biology · 2019
    Review
  14. Hyaluronan biology: A complex balancing act of structure, function, location and context.Matrix biology : journal of the International Society for Matrix Biology · 2019
    Review
  15. Review
  16. The journey of hyaluronan research in theThe Journal of biological chemistry · 2019
    Review
  17. Review
  18. HYAL-2-WWOX-SMAD4 Signaling in Cell Death and Anticancer Response.Frontiers in cell and developmental biology · 2016
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Aaron C PetreyDepartment of Pathobiology, Cleveland Clinic Lerner Research Institute, Cleveland, Ohio.
Dana R OberyDepartment of Pathobiology, Cleveland Clinic Lerner Research Institute, Cleveland, Ohio.
Sean P KesslerDepartment of Pathobiology, Cleveland Clinic Lerner Research Institute, Cleveland, Ohio.
Bruno FlamionMolecular Physiology Research Unit, Namur Research Institute for the Life Sciences, University of Namur, Namur, Belgium.
Carol A de la MotteDepartment of Pathobiology, Cleveland Clinic Lerner Research Institute, Cleveland, Ohio. Electronic address: delamoc@ccf.org.
Cleveland Clinic Lerner College of Medicine · USUniversity of Namur · BE

Funding

VERSICAN PROTEOLYSIS AND REGULATION OF VASCULAR SMOOTH MUSCLEP01HL107147 · NHLBI · CLEVELAND CLINIC LERNER COM-CWRU · PI HASCALL, VINCENT CHARLES · 2011 to 2017
$16.1M
NHLBI NIH HHS P01 HL107147
6 · The paper itself

Abstract

Hyaluronan is the predominant glycosaminoglycan component of the extracellular matrix with an emerging role in hematopoiesis. Modulation of hyaluronan polymer size is responsible for its control over cellular functions, and the balance of hyaluronan synthesis and degradation determines its molecular size. Although two active somatic hyaluronidases are expressed in mammals, only deficiency in hyaluronidase-2 (Hyal-2) results in thrombocytopenia of unknown mechanism. Our results reveal that Hyal-2 knockout mice accumulate hyaluronan within their bone marrow and within megakaryocytes, the cells responsible for platelet generation. Proplatelet formation by Hyal-2 knockout megakaryocytes was disrupted because of abnormal formation of the demarcation membrane system, which was dilated and poorly developed. Importantly, peptide-mediated delivery of exogenous hyaluronidase rescued deficient proplatelet formation in murine and human megakaryocytes lacking Hyal-2. Together, our data uncover a previously unsuspected mechanism of how hyaluronan and Hyal-2 control platelet generation.

Indexed as

AnimalsApoptosisHumansHyaluronic AcidHyaluronoglucosaminidaseImmunoblottingImmunohistochemistryIn Situ Nick-End LabelingMegakaryocytesMiceMice, KnockoutMicroscopy, Electron, TransmissionReal-Time Polymerase Chain ReactionThrombopoiesisHyaluronic AcidHyaluronoglucosaminidase

Identifiers

PMID27398974
PMCPMC5012466
OpenAlexW2469002652

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.