Evidence map›Paper›PMID 27398792›Full record

ArticleNature biotechnology2016

A recellularized human colon model identifies cancer driver genes.

Huanhuan Joyce Chen, Zhubo Wei, Jian Sun, Asmita Bhattacharya, David J Savage, Rita Serda, Yuri Mackeyev, Steven A Curley, Pengcheng Bu, Lihua Wang and 8 more

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 59 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
59citing papers in PubMed, 1 pooled it
7.6field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

59 citing papers in PubMed, 1 synthesis or guideline pooled it, 110 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Potential Use of Organoids in Regenerative Medicine.Tissue engineering and regenerative medicine · 2024
    Review
  4. Article
  5. Advancements in 3D In Vitro Models for Colorectal Cancer.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024
    Review
  6. Article
  7. Review
  8. Review
  9. Article
  10. Anti-Cancer Effect ofReports of biochemistry & molecular biology · 2023
    Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Review
  16. Review
  17. Review
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

18 authors at 5 institutions in 1 country.

Huanhuan Joyce ChenDepartment of Biomedical Engineering, Cornell University, Ithaca, New York, USA.
Zhubo WeiCancer Research Program, Houston Methodist Research Institute, Houston, Texas, USA.
Jian SunDepartment of Medicine, Weill Cornell Medical College, New York, New York, USA.
Asmita BhattacharyaGenetics, Genomics and Development, Cornell University, Ithaca, New York, USA.
David J SavageUniversity of Texas Medical School at Houston, Houston, Texas, USA.
Rita SerdaUniversity of Texas Medical School at Houston, Houston, Texas, USA.
Yuri MackeyevDepartment of Chemistry, Rice University, Houston, Texas, USA.
Steven A CurleyDepartment of Surgery, Baylor College of Medicine, Houston, Texas, USA.
Pengcheng BuDepartment of Biomedical Engineering, Cornell University, Ithaca, New York, USA.
Lihua WangDepartment of Biological and Environmental Engineering, Cornell University, Ithaca, New York, USA.
Shuibing ChenChemical Biology in Surgery, Weill Cornell Medical College, New York, New York, USA.
Leona Cohen-GouldDepartment of Biochemistry, Weill Cornell Medical College, New York, New York, USA.
Emina HuangDepartment of Stem Cell Biology and Regenerative Medicine, Cleveland Clinic, Cleveland, Ohio, USA.
Xiling ShenDepartment of Biomedical Engineering, Cornell University, Ithaca, New York, USA.
Steven M LipkinDepartment of Medicine, Weill Cornell Medical College, New York, New York, USA.
Neal G CopelandCancer Research Program, Houston Methodist Research Institute, Houston, Texas, USA.
Nancy A JenkinsCancer Research Program, Houston Methodist Research Institute, Houston, Texas, USA.
Michael L ShulerDepartment of Biomedical Engineering, Cornell University, Ithaca, New York, USA.
Cornell University · USHouston Methodist · USBaylor College of Medicine · USCleveland Clinic · USRice University · US

Funding

University of California Health Participation in the National COVID Cohort Collaborative (N3C)UL1TR001414 · NCATS · UNIVERSITY OF CALIFORNIA-IRVINE · PI COOPER, DAN M, VILAIN, ERIC J. · 2015 to 2023
$35.1M
Microphysiologial systems and low cost microfluidic platform with analyticsUH2TR000516 · NCATS · CORNELL UNIVERSITY · PI HICKMAN, JAMES J, SHULER, MICHAEL L · 2012 to 2014
$2.9M
The role of IL8 in colitis-associated tumor initiationR01CA157663 · NCI · UNIVERSITY OF FLORIDA · PI HUANG, EMINA HUI-NA · 2012 to 2016
$1.6M
Multi-scale modeling of asymmetric cell divisionR01GM095990 · NIGMS · CORNELL UNIVERSITY · PI SHEN, XILING · 2011 to 2015
$1.5M
Robustness of the Intestinal Stem Cell NicheR01GM114254 · NIGMS · DUKE UNIVERSITY · PI SHEN, XILING · 2015 to 2017
$918k
NCATS NIH HHS UH2 TR000516NCATS NIH HHS UL1 TR001414NCI NIH HHS R01 CA157663NIGMS NIH HHS R01 GM095990NIGMS NIH HHS R01 GM114254
6 · The paper itself

Abstract

Refined cancer models are needed to bridge the gaps between cell line, animal and clinical research. Here we describe the engineering of an organotypic colon cancer model by recellularization of a native human matrix that contains cell-populated mucosa and an intact muscularis mucosa layer. This ex vivo system recapitulates the pathophysiological progression from APC-mutant neoplasia to submucosal invasive tumor. We used it to perform a Sleeping Beauty transposon mutagenesis screen to identify genes that cooperate with mutant APC in driving invasive neoplasia. We identified 38 candidate invasion-driver genes, 17 of which, including TCF7L2, TWIST2, MSH2, DCC, EPHB1 and EPHB2 have been previously implicated in colorectal cancer progression. Six invasion-driver genes that have not, to our knowledge, been previously described were validated in vitro using cell proliferation, migration and invasion assays and ex vivo using recellularized human colon. These results demonstrate the utility of our organoid model for studying cancer biology.

Indexed as

Adenomatous Polyposis Coli ProteinCarcinogenesisCell-Free SystemCells, CulturedColonColonic NeoplasmsGene Expression ProfilingGenes, NeoplasmHumansNeoplasm ProteinsOrganogenesisTissue EngineeringAdenomatous Polyposis Coli ProteinAPC protein, humanNeoplasm Proteins

Identifiers

PMID27398792
PMCPMC4980997
OpenAlexW2474578758

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.