ArticleJournal of stem cells & regenerative medicine2016
In vitro study of the role of thrombin in platelet rich plasma (PRP) preparation: utility for gel formation and impact in growth factors release.
Article in Journal of stem cells & regenerative medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 39 citations in OpenAlex.
- Healing effects of autologous platelet gel and growth factors on cutaneous leishmaniasis wounds in addition to antimony; a self-controlled clinical trial with randomized lesion assignment.BMC research notes · 2023Trial
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- Arthroscopic PRP gel augmentation at the tendon-bone interface accelerates functional recovery and structural healing in rotator cuff repair: a retrospective cohort study.BMC musculoskeletal disorders · 2025Article
- Platelet-rich plasma as a potential antimicrobial agent against multidrug-resistant bacteria in diabetic foot infections.Scientific reports · 2025Article
- Modulating rheology and bioactivity in dermal fillers: the emerging role of platelet-rich plasma (PRP).Frontiers in medicine · 2025Review
- Review
- Role of platelet rich plasma mediated repair and regeneration of cell in early stage of cardiac injury.Regenerative therapy · 2022Review
- Characterization of autologous platelet rich plasma (PRP) and its biological effects in patients with Behçet's Disease.Regenerative therapy · 2021Article
- Orthobiologics in the treatment of hip disorders.World journal of stem cells · 2021Review
- Potential of Lyophilized Platelet Concentrates for Craniofacial Tissue Regenerative Therapies.Molecules (Basel, Switzerland) · 2021Review
- Rheological Properties and Growth Factors Content of Platelet-Rich Plasma: Relevance in Veterinary Biomedical Treatments.Biomedicines · 2020Article
- Platelet-Rich Plasma for the Treatment of Tissue Infection: Preparation and Clinical Evaluation.Tissue engineering. Part B, Reviews · 2019Review
- Variables affecting the potential efficacy of PRP in providing chronic pain relief.Journal of pain research · 2019Review
- Platelet-rich Plasma Protects HUVECs against oX-LDL-induced Injury.Open medicine (Warsaw, Poland) · 2018Article
- Antibacterial Activity of Leukocyte- and Platelet-Rich Plasma: AnBioMed research international · 2018Article
- Platelet-Rich Plasma Derived Growth Factors Contribute to Stem Cell Differentiation in Musculoskeletal Regeneration.Frontiers in chemistry · 2017Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionThe use of PRP has been studied for different fields, with promising results in regenerative medicine. Until now, there is no study in the literature evaluating thrombin levels in serum, used as autologous thrombin preparation. Therefore, in the present study we evaluated the role played by different thrombin concentrations in PRP and the impact in the release of growth factors. Also, different activators for PRP gel formation were evaluated.
methodsThrombin levels were measured in different autologous preparations: serum, L-PRP (PRP rich in leukocytes) and T-PRP (thrombin produced through PRP added calcium gluconate). L-PRP was prepared according to the literature, with platelets and leukocytes being quantified. The effect of autologous thrombin associated or not with calcium in PRP gel was determined by measuring the time of gel formation. The relationship between thrombin concentration and release of growth factors was determined by growth factors (PDGF-AA, VEGF and EGF) multiplex analysis.
resultsA similar concentration of thrombin was observed in serum, L-PRP and T-PRP (8.13 nM, 8.63 nM and 7.56 nM, respectively) with a high variation between individuals (CV%: 35.07, 43 and 58.42, respectively). T-PRP and serum with calcium chloride showed similar results in time to promote gel formation. The increase of thrombin concentrations (2.66, 8 and 24 nM) did not promote an increase in growth factor release.
conclusionsThe technique of using serum as a thrombin source proved to be the most efficient and reproducible for promoting PRP gel formation, with some advantages when compared to other activation methods, as this technique is easier and quicker with no need of consuming part of PRP. Noteworthy, PRP activation using different thrombin concentrations did not promote a higher release of growth factors, appearing not to be necessary when PRP is used as a suspension.
Indexed as
Identifiers
27397996PMC4929890W2477601863What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.