Evidence map›Paper›PMID 27397520›Full record

ArticleNeuron2016

Real-Time Imaging Reveals Properties of Glutamate-Induced Arc/Arg 3.1 Translation in Neuronal Dendrites.

Youn Na, Sungjin Park, Changhee Lee, Dong-Kyu Kim, Joo Min Park, Shanthini Sockanathan, Richard L Huganir, Paul F Worley

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuron, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed
4.9field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed, 65 citations in OpenAlex.

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  19. Homeostatic Roles of the Proteostasis Network in Dendrites.Frontiers in cellular neuroscience · 2020
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 3 countries.

Youn NaSolomon Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Divison of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
Sungjin ParkSolomon Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Neurobiology and Anatomy, University of Utah, Salt Lake City, UT 84132, USA.
Changhee LeeSolomon Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Genetics, Harvard Medical School, Boston, MA 02446, USA.
Dong-Kyu KimDepartment of Neurobiology and Anatomy, University of Utah, Salt Lake City, UT 84132, USA.
Joo Min ParkSolomon Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Center for Cognition and Sociality, Institute for Basic Science, Daejeon 305-811, Korea.
Shanthini SockanathanSolomon Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Richard L HuganirSolomon Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Paul F WorleySolomon Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. Electronic address: pworley@jhmi.edu.
Johns Hopkins University · USCape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa · ZAHarvard University · USUniversity of Utah · US

Funding

NOVEL CELLULAR MECHANISMS IN CORTEX DEVELOPMENTR01MH053608 · NIMH · JOHNS HOPKINS UNIVERSITY · PI WORLEY, PAUL F · 1994 to 2024
$9.3M
Signaling to and from the SynapseP50MH084020 · NIMH · JOHNS HOPKINS UNIVERSITY · PI HUGANIR, RICHARD L · 2008 to 2012
$9.2M
NIMH NIH HHS P50 MH084020NIMH NIH HHS R01 MH053608
6 · The paper itself

Abstract

The immediate early gene Arc (also Arg3.1) produces rapid changes in synaptic properties that are linked to de novo translation. Here we develop a novel translation reporter that exploits the rapid maturation and "flash" kinetics of Gaussia luciferase (Gluc) to visualize Arc translation. Following glutamate stimulation, discrete Arc-Gluc bioluminescent flashes representing sites of de novo translation are detected within 15 s at distributed sites in dendrites, but not spines. Flashes are episodic, lasting ∼20 s, and may be unitary or repeated at ∼minute intervals at the same sites. Analysis of flash amplitudes suggests they represent the quantal product of one or more polyribosomes, while inter-flash intervals appear random, suggesting they arise from a stochastic process. Surprisingly, glutamate-induced translation is dependent on Arc open reading frame. Combined observations support a model in which stalled ribosomes are reactivated to rapidly generate Arc protein.

Indexed as

Protein BiosynthesisAnimalsCells, CulturedCytoskeletal ProteinsDendritesGene Expression RegulationGlutamic AcidLuminescent MeasurementsNerve Tissue ProteinsRatsRats, Sprague-Dawleyactivity regulated cytoskeletal-associated proteinCytoskeletal ProteinsGlutamic AcidNerve Tissue Proteins

Identifiers

PMID27397520
PMCPMC5580817
OpenAlexW2469423203

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.