Evidence map›Paper›PMID 27394413›Full record

ArticleJournal of molecular medicine (Berlin, Germany)2016

Circulating adipocyte-derived extracellular vesicles are novel markers of metabolic stress.

Akiko Eguchi, Milos Lazic, Aaron M Armando, Susan A Phillips, Roia Katebian, Spyridoula Maraka, Oswald Quehenberger, Dorothy D Sears, Ariel E Feldstein

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of molecular medicine (Berlin, Germany), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 121 papers.

0numbers the graph read from it
0cells of the map it votes in
121citing papers in PubMed
5.5field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

121 citing papers in PubMed, 184 citations in OpenAlex.

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61 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 1 country.

Akiko EguchiDepartment of Pediatrics, University of California San Diego (UCSD), La Jolla, CA, 92093, USA.
Milos LazicDepartment of Pediatrics, University of California San Diego (UCSD), La Jolla, CA, 92093, USA.
Aaron M ArmandoDepartment of Pharmacology, University of California San Diego (UCSD), La Jolla, CA, 92093, USA.
Susan A PhillipsDepartment of Pediatrics, University of California San Diego (UCSD), La Jolla, CA, 92093, USA.
Roia KatebianDepartment of Pediatrics, Mount Sinai Hospital, Chicago, IL, 60608, USA.
Spyridoula MarakaDepartment of Internal Medicine, University of Connecticut School of Medicine, Farmington, CT, 06030, USA.
Oswald QuehenbergerDepartment of Pharmacology, University of California San Diego (UCSD), La Jolla, CA, 92093, USA.
Dorothy D SearsDivision of Endocrinology and Metabolism, University of California San Diego (UCSD), La Jolla, CA, 92093, USA.
Ariel E FeldsteinDepartment of Pediatrics, University of California San Diego (UCSD), La Jolla, CA, 92093, USA. afeldstein@ucsd.edu.
University of California, San Diego · USMount Sinai Hospital · USRady Children's Hospital-San Diego · USUniversity of Connecticut · US

Funding

Oxidized Metabolites of Linoleic Acid in Alcohol-induced Liver InjuryU01AA022489 · NIAAA · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI FELDSTEIN, ARIEL, MCCLAIN, CRAIG J. · 2014 to 2018
$1.7M
Caspase Dependent Cell Death in Nonalcoholic Fatty Liver DiseaseR01DK082451 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI FELDSTEIN, ARIEL · 2009 to 2012
$1.4M
Encapsulated RNAs in extracellular vesicles contribute ALD progressionR21AA023574 · NIAAA · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI EGUCHI, AKIKO, FELDSTEIN, ARIEL · 2015 to 2016
$407k
NIAAA NIH HHS R21 AA023574NIAAA NIH HHS U01 AA022489NIDDK NIH HHS R01 DK082451
6 · The paper itself

Abstract

We recently reported that stressed adipocytes release extracellular vesicles (EVs) that act as "find-me" signals to promote macrophage migration and activation. In this study, we performed a comprehensive characterization of stressed adipocyte-derived EVs, assessing their antigenic composition, lipidomics, and RNA profiles. Perilipin A was identified as one of the adipose-specific proteins and studied as a potential novel biomarker to detect adipocyte-derived EVs in circulation. Circulating EVs were significantly increased in mice with diet-induced obesity (DIO) and in obese humans with metabolic syndrome compared to lean controls. This increase was associated with decreased glucose tolerance in the DIO mice and metabolic dysfunction, elevated insulin, and homeostatic model assessment of insulin resistance (HOMA-IR) in the obese humans. EVs from both DIO mice and obese humans were enriched in perilipin A, a central gatekeeper of the adipocyte lipid storehouse and a marker of adipocyte differentiation. In obese humans, circulating levels of EVs enriched in perilipin A were dynamic, decreasing 35 % (p < 0.05) after a 3-month reduced calorie diet intervention. This translational study provides an extensive characterization of adipocyte-derived EVs. The findings identify perilipin A as a novel biomarker of circulating EVs of adipocyte origin and support the development of circulating perilipin A-positive EVs as indicators of adipose tissue health. KEY MESSAGE: • Extensive characterization of 3T3L1 EVs identified perilipin A in their composition. • Circulating EVs are elevated in obese mice and associated with glucose intolerance. • Circulating EVs are elevated in obese human and correlated with metabolic factors. • Perilipin A and EV levels are increased in the circulation of obese mice and human. • Circulating EV and perilipin A levels decrease with low calorie intervention.

Indexed as

Stress, Physiological3T3-L1 CellsAdipocytesAdultAnimalsBiomarkersCaloric RestrictionCells, CulturedExtracellular VesiclesHumansInsulinInsulin ResistanceLipidsMetabolomicsMiceMice, Inbred C57BLBiomarkersInsulinLipidsMicroRNAsPerilipin-1ProteinsAdipocyte stress and hypertrophyExtracellular vesiclesNoninvasive biomarkerObesity

Identifiers

PMID27394413
PMCPMC5071132
OpenAlexW2464729198

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.