Evidence map›Paper›PMID 27393496›Full record

ArticleHistochemistry and cell biology2016

Differential expression and localization of Ankrd2 isoforms in human skeletal and cardiac muscles.

Jovana Jasnic-Savovic, Sabine Krause, Slobodan Savic, Ana Kojic, Vlado Kovcic, Srdjan Boskovic, Aleksandra Nestorovic, Ljiljana Rakicevic, Olivia Schreiber-Katz, Johannes G Vogel and 6 more

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Article in Histochemistry and cell biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.7field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 3 institutions in 4 countries.

Jovana Jasnic-SavovicInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO Box 23, Belgrade, 11010, Serbia.
Sabine KrauseDepartment of Neurology, Friedrich Baur Institute, Ludwig Maximilians University, Ziemssenstr. 1a, 80336, Munich, Germany.
Slobodan SavicInstitute of Forensic Medicine, School of Medicine, University of Belgrade, Deligradska 31a, Belgrade, 11000, Serbia.
Ana KojicInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO Box 23, Belgrade, 11010, Serbia.
Vlado KovcicInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO Box 23, Belgrade, 11010, Serbia.
Srdjan BoskovicInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO Box 23, Belgrade, 11010, Serbia.
Aleksandra NestorovicInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO Box 23, Belgrade, 11010, Serbia.
Ljiljana RakicevicInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO Box 23, Belgrade, 11010, Serbia.
Olivia Schreiber-KatzDepartment of Neurology, Friedrich Baur Institute, Ludwig Maximilians University, Ziemssenstr. 1a, 80336, Munich, Germany.
Johannes G VogelDepartment of Neurology, Friedrich Baur Institute, Ludwig Maximilians University, Ziemssenstr. 1a, 80336, Munich, Germany.
Benedikt G SchoserDepartment of Neurology, Friedrich Baur Institute, Ludwig Maximilians University, Ziemssenstr. 1a, 80336, Munich, Germany.
Maggie C WalterDepartment of Neurology, Friedrich Baur Institute, Ludwig Maximilians University, Ziemssenstr. 1a, 80336, Munich, Germany.
Giorgio ValleCRIBI, University of Padova, Via Ugo Bassi 58b, 35121, Padua, Italy.
Dragica RadojkovicInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO Box 23, Belgrade, 11010, Serbia.
Georgine FaulknerCRIBI, University of Padova, Via Ugo Bassi 58b, 35121, Padua, Italy.
Snezana KojicInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO Box 23, Belgrade, 11010, Serbia. snezanakojic@imgge.bg.ac.rs.ORCID http://orcid.org/0000-0001-5090-1278
University of Belgrade · RSFriedrich Baur Stiftung · DEUniversity of Padua · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Four human Ankrd2 transcripts, reported in the Ensembl database, code for distinct protein isoforms (360, 333, 327 and 300 aa), and so far, their existence, specific expression and localization patterns have not been studied in detail. Ankrd2 is preferentially expressed in the slow fibers of skeletal muscle. It is found in both the nuclei and the cytoplasm of skeletal muscle cells, and its localization is prone to change during differentiation and upon stress. Ankrd2 has also been detected in the heart, in ventricular cardiomyocytes and in the intercalated disks (ICDs). The main objective of this study was to distinguish between the Ankrd2 isoforms and to determine the contribution of each one to the general profile of Ankrd2 expression in striated muscles. We demonstrated that the known expression and localization pattern of Ankrd2 in striated muscle can be attributed to the isoform of 333 aa which is dominant in both tissues, while the designated cardiac and canonical isoform of 360 aa was less expressed in both tissues. The 360 aa isoform has a distinct nuclear localization in human skeletal muscle, as well as in primary myoblasts and myotubes. In contrast to the isoform of 333 aa, it was not preferentially expressed in slow fibers and not localized to the ICDs of human cardiomyocytes. Regulation of the expression of both isoforms is achieved at the transcriptional level. Our results set the stage for investigation of the specific functions and interactions of the Ankrd2 isoforms in healthy and diseased human striated muscles.

Indexed as

Amino Acid SequenceCells, CulturedHumansMuscle ProteinsMuscle, SkeletalMyocardiumNuclear ProteinsProtein IsoformsRepressor ProteinsSequence AlignmentANKRD2 protein, humanMuscle ProteinsNuclear ProteinsProtein IsoformsRepressor ProteinsAnkrd2HeartHumanIsoformsSkeletal muscle

Identifiers

PMID27393496
OpenAlexW2463693196

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.