Evidence map›Paper›PMID 27393332›Full record

ArticleGeneral thoracic and cardiovascular surgery2016

Association between endothelial function and micro-vascular remodeling measured by synchrotron radiation pulmonary micro-angiography in pulmonary arterial hypertension.

Satoko Fuji, Shonosuke Matsushita, Kazuyuki Hyodo, Motoo Osaka, Hiroaki Sakamoto, Kenkichi Tanioka, Kazunori Miyakawa, Misao Kubota, Yuji Hiramatsu, Chiho Tokunaga

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Article in General thoracic and cardiovascular surgery, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.8field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Satoko FujiSchool of Medicine, University of Tsukuba, Tsukuba, Japan.
Shonosuke MatsushitaDepartment of Health Science, Tsukuba University of Technology, Tsukuba, Japan.
Kazuyuki HyodoPhoton Factory, High Energy Accelerator Research Organization, Tsukuba, Japan.
Motoo OsakaDepartment of Cardiovascular Surgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, 305-8577, Japan.
Hiroaki SakamotoDepartment of Cardiovascular Surgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, 305-8577, Japan.
Kenkichi TaniokaTokyo Denki University, Tsukuba, Japan.
Kazunori MiyakawaNHK Science and Technology Research Laboratories, Tsukuba, Japan.
Misao KubotaNHK Science and Technology Research Laboratories, Tsukuba, Japan.
Yuji HiramatsuDepartment of Cardiovascular Surgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, 305-8577, Japan.
Chiho TokunagaDepartment of Cardiovascular Surgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, 305-8577, Japan. chiho-t@md.tsukuba.ac.jp.ORCID http://orcid.org/0000-0002-1349-4148
University of Tsukuba · JPHigh Energy Accelerator Research Organization · JPTokyo Denki University · JPTsukuba University of Technology · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesPulmonary arterial hypertension (PAH) is a progressive disease which causes increased vascular resistance. In this study, our purpose was to quantify the micro-vascular remodeling in monocrotaline-induced PAH rats using synchrotron radiation pulmonary micro-angiography (SRPA), a method we have previously established in an in vivo rat model. To determine the relationship between endothelial function and vascular remodeling, the local expression of endothelin-1 (ET-1), endothelial nitric oxide synthase (eNOS) and vascular endothelial growth factor (VEGF) was evaluated using immunohistochemical staining.

methodsMonocrotaline-induced PAH rats were created by subcutaneous injection of monocrotaline. After 2 weeks, SRPA was performed at the Photon Factory of the High Energy Accelerator Research Organization. The internal diameters of pulmonary arterioles were measured using SRPA images. Semi-quantified analyses of ET-1, eNOS and VEGF expression in pulmonary arterioles were performed by immunohistochemical staining.

resultsMicro-vascular density and the internal diameters of pulmonary arterioles were significantly decreased in PAH. ET-1 expression was significantly increased in PAH compared with the control (1.53 ± 0.45 vs. 0.80 ± 0.14) and eNOS expression was significantly decreased in PAH compared with the control (1.12 ± 0.59 vs. 1.91 ± 0.66), although VEGF expression did not differ between the groups.

conclusionsSRPA can be effectively used for visualizing the decreased pulmonary micro-vasculature associated with PAH. Increased ET-1 expression and decreased eNOS expression may contribute to the proliferation and vasospasm of pulmonary arterioles induced by endothelial dysfunction due to PAH. This SRPA technology may help to identify a correlation between endothelial function and micro-vasculature remodeling in PAH.

Indexed as

AngiographyAnimalsArterial PressureArteriolesEndothelium, VascularHypertension, PulmonaryHypertrophyLungMaleMonocrotalineNitric Oxide Synthase Type IIIPulmonary ArteryRatsRats, Sprague-DawleySynchrotronsThrombosisMonocrotalineNitric Oxide Synthase Type IIINOS3 protein, humanVascular Endothelial Growth Factor AMicro-vascular remodelingPulmonary artery hypertensionSynchrotron radiation

Identifiers

PMID27393332
OpenAlexW2468405064

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.