ArticleJournal of neuroscience methods2016
Two-photon imaging of cerebral hemodynamics and neural activity in awake and anesthetized marmosets.
Article in Journal of neuroscience methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 57 citations in OpenAlex.
- Article
- Arteries and veins in awake mice using two-photon microscopy.Journal of anatomy · 2025Article
- Neural Recording Chambers for Long-Term Access to Brain Tissue.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Arterial and venous flow dynamics are modified by age in the non-human primate.Imaging neuroscience (Cambridge, Mass.) · 2025Article
- Altered projection-specific synaptic remodeling and its modification by oxytocin in an idiopathic autism marmoset model.Communications biology · 2024Article
- Mesoscopic calcium imaging in a head-unrestrained male non-human primate using a lensless microscope.Nature communications · 2024Article
- Genetic Approaches for Neural Circuits Dissection in Non-human Primates.Neuroscience bulletin · 2023Review
- Large-volume and deep brain imaging in rabbits and monkeys using COMPACT two-photon microscopy.Scientific reports · 2022Article
- Review
- Advanced high resolution three-dimensional imaging to visualize the cerebral neurovascular network in stroke.International journal of biological sciences · 2022Review
- Marmosets: a promising model for probing the neural mechanisms underlying complex visual networks such as the frontal-parietal network.Brain structure & function · 2021Review
- Miniature microscopes for manipulating and recording in vivo brain activity.Microscopy (Oxford, England) · 2021Article
- Head-mounted microendoscopic calcium imaging in dorsal premotor cortex of behaving rhesus macaque.Cell reports · 2021Article
- Differences in cerebral blood vasculature and flow in awake and anesthetized mouse cortex revealed by quantitative optical coherence tomography angiography.Journal of neuroscience methods · 2021Article
- Using non-invasive neuroimaging to enhance the care, well-being and experimental outcomes of laboratory non-human primates (monkeys).NeuroImage · 2021Review
- Tonic regulation of middle meningeal artery diameter by ATP-sensitive potassium channels.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2019Article
- Generation of genetically engineered non-human primate models of brain function and neurological disorders.American journal of primatology · 2019Review
- Comparative Principles for Next-Generation Neuroscience.Frontiers in behavioral neuroscience · 2019Article
- Emerging ideas and tools to study the emergent properties of the cortical neural circuits for voluntary motor control in non-human primates.F1000Research · 2019Review
- Mannose-Binding Lectin Drives Platelet Inflammatory Phenotype and Vascular Damage After Cerebral Ischemia in Mice via IL (Interleukin)-1α.Arteriosclerosis, thrombosis, and vascular biology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundMarmosets are a powerful, emerging model for human behavior and neurological disorders. However, longitudinal imaging modalities that visualize both cellular structure and function within the cortex are not available in this animal model. Hence, we implemented an approach to quantify vascular topology, hemodynamics, and neural activity in awake marmosets using two-photon microscopy (2PM). NEW
methodMarmosets were acclimated to a custom stereotaxic system. AAV1-GCaMP5G was injected into somatosensory cortex to optically indicate neural activity, and a cranial chamber was implanted.
resultsLongitudinal 2PM revealed vasculature and neurons 500μm below the cortical surface. Vascular response and neural activity during sensory stimulation were preserved over 5 and 3 months, respectively, before optical quality deteriorated. Vascular remodeling including increased tortuosity and branching was quantified. However, capillary connectivity from arterioles to venules remained unchanged. Further, behavioral assessment before and after surgery demonstrated no impact on cognitive and motor function. Immunohistochemistry confirmed minimal astrocyte activation with no focal damage. Over 6 months, total cortical depth visualized decreased. When under anesthesia, the most prominent isoflurane-induced vasodilation occurred in capillaries and smaller arterioles. COMPARISON WITH EXISTING METHOD(S): These results demonstrate the capability to repeatedly observe cortical physiology in awake marmosets over months.
conclusionsThis work provides a novel and insightful technique to investigate critical mechanisms in neurological disorders in awake marmosets without introducing confounds from anesthesia.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.