ArticleEuropean journal of immunology2016
Expression of heavy chain-only antibodies can support B-cell development in light chain knockout chickens.
Article in European journal of immunology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 42 citations in OpenAlex.
- Deciphering interferon functions in avian influenza using receptor knockout models in the natural host.eLife · 2026Article
- Advancing immunity and disease resistance in chickens through genome editing.Journal of animal science and biotechnology · 2026Review
- Unique chicken B cell development: species-specific mechanisms and contradictory requirements of B cell receptor for post-hatched B cell development.Frontiers in immunology · 2026Review
- Genome Editing in the Chicken: From PGC-Mediated Germline Transmission to Advanced Applications.International journal of molecular sciences · 2025Review
- Characterization of class-switched B cells in chickens.Frontiers in immunology · 2024Article
- Comparison of Multiple Strategies for Precision Transgene Knock-In inInternational journal of molecular sciences · 2023Article
- Review
- Development and characterization of a CRISPR/Cas9-mediatedFrontiers in immunology · 2022Article
- Chickens, more than humans, focus the diversity of their immunoglobulin genes on the complementarity-determining region but utilise amino acids, indicative of a more cross-reactive antibody repertoire.Frontiers in immunology · 2022Article
- Highly Efficient Genome Modification of Cultured Primordial Germ Cells with Lentiviral Vectors to Generate Transgenic Songbirds.Stem cell reports · 2021Article
- Cas9-expressing chickens and pigs as resources for genome editing in livestock.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Direct allele introgression into pure chicken breeds using Sire Dam Surrogate (SDS) mating.Nature communications · 2021Article
- Targeting the myeloid checkpoint receptor SIRPα potentiates innate and adaptive immune responses to promote anti-tumor activity.Journal of hematology & oncology · 2020Article
- Current Approaches and Applications in Avian Genome Editing.International journal of molecular sciences · 2020Review
- Isolation of rabbit single domain antibodies to B7-H3 via protein immunization and phage display.Antibody therapeutics · 2020Article
- Article
- Applications of Gene Editing in Chickens: A New Era Is on the Horizon.Frontiers in genetics · 2018Review
- Article
- Genetic Removal of the CH1 Exon Enables the Production of Heavy Chain-Only IgG in Mice.Frontiers in immunology · 2018Article
- Male fertility restored by transplanting primordial germ cells into testes: a new way towards efficient transgenesis in chicken.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
Abstract
Since the discovery of antibody-producing B cells in chickens six decades ago, chickens have been a model for B-cell development in gut-associated lymphoid tissue species. Here we describe targeting of the immunoglobulin light chain locus by homologous recombination in chicken primordial germ cells (PGCs) and generation of VJCL knockout chickens. In contrast to immunoglobulin heavy chain knockout chickens, which completely lack mature B cells, homozygous light chain knockout (IgL(-/-) ) chickens have a small population of B lineage cells that develop in the bursa and migrate to the periphery. This population of B cells expresses the immunoglobulin heavy chain molecule on the cell surface. Soluble heavy-chain-only IgM and IgY proteins of reduced molecular weight were detectable in plasma in 4-week-old IgL(-/-) chickens, and antigen-specific IgM and IgY heavy chain proteins were produced in response to immunization. Circulating heavy-chain-only IgM showed a deletion of the CH1 domain of the constant region enabling the immunoglobulin heavy chain to be secreted in the absence of the light chain. Our data suggest that the heavy chain by itself is enough to support all the important steps in B-cell development in a gut-associated lymphoid tissue species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.