ArticleBMC biology2016
A new role of hindbrain boundaries as pools of neural stem/progenitor cells regulated by Sox2.
Article in BMC biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 42 citations in OpenAlex.
- Maternal Exposure to Carbamazepine at Environmentally Relevant Concentrations Causes Growth Delay in Mouse Embryos.ACS omega · 2025Article
- Her9 controls the stemness properties of hindbrain boundary cells.Development (Cambridge, England) · 2025Article
- Endolysosomal dysfunction in radial glia progenitor cells leads to defective cerebral angiogenesis and compromised blood-brain barrier integrity.Nature communications · 2024Article
- Hindbrain boundaries as niches of neural progenitor and stem cells regulated by the extracellular matrix proteoglycan chondroitin sulphate.Development (Cambridge, England) · 2024Article
- Imaging and multi-omics datasets converge to define different neural progenitor origins for ATRT-SHH subgroups.Nature communications · 2023Article
- Zbtb16 mediates a switch between Fgf signalling regimes in the developing hindbrain.Development (Cambridge, England) · 2023Article
- Hindbrain rhombomere centers harbor a heterogenous population of dividing progenitors which rely on Notch signaling.Frontiers in cell and developmental biology · 2023Article
- Segmentation and patterning of the vertebrate hindbrain.Development (Cambridge, England) · 2021Review
- Forced to communicate: Integration of mechanical and biochemical signaling in morphogenesis.Current opinion in cell biology · 2020Review
- Temporal-specific roles of fragile X mental retardation protein in the development of the hindbrain auditory circuit.Development (Cambridge, England) · 2020Article
- A single cell transcriptome atlas of the developing zebrafish hindbrain.Development (Cambridge, England) · 2020Article
- SOX2 regulates homeostasis of taste bud cells and lingual epithelial cells in posterior tongue.PloS one · 2020Article
- The interplay of atoh1 genes in the lower rhombic lip during hindbrain morphogenesis.PloS one · 2020Article
- Actomyosin regulation by Eph receptor signaling couples boundary cell formation to border sharpness.eLife · 2019Article
- The role of PHOX2B-derived astrocytes in chemosensory control of breathing and sleep homeostasis.The Journal of physiology · 2019Article
- Hindbrain induction and patterning during early vertebrate development.Cellular and molecular life sciences : CMLS · 2019Review
- Cellular organization and boundary formation in craniofacial development.Genesis (New York, N.Y. : 2000) · 2019Review
- Neural stem cells deriving from chick embryonic hindbrain recapitulate hindbrain development in culture.Scientific reports · 2018Article
- Evolutionary emergence of theProceedings of the National Academy of Sciences of the United States of America · 2018Article
- The Mouse Hindbrain As a Model for Studying Embryonic Neurogenesis.Journal of visualized experiments : JoVE · 2018Article
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCompartment boundaries are an essential developmental mechanism throughout evolution, designated to act as organizing centers and to regulate and localize differently fated cells. The hindbrain serves as a fascinating example for this phenomenon as its early development is devoted to the formation of repetitive rhombomeres and their well-defined boundaries in all vertebrates. Yet, the actual role of hindbrain boundaries remains unresolved, especially in amniotes.
resultsHere, we report that hindbrain boundaries in the chick embryo consist of a subset of cells expressing the key neural stem cell (NSC) gene Sox2. These cells co-express other neural progenitor markers such as Transitin (the avian Nestin), GFAP, Pax6 and chondroitin sulfate proteoglycan. The majority of the Sox2(+) cells that reside within the boundary core are slow-dividing, whereas nearer to and within rhombomeres Sox2(+) cells are largely proliferating. In vivo analyses and cell tracing experiments revealed the contribution of boundary Sox2(+) cells to neurons in a ventricular-to-mantle manner within the boundaries, as well as their lateral contribution to proliferating Sox2(+) cells in rhombomeres. The generation of boundary-derived neurospheres from hindbrain cultures confirmed the typical NSC behavior of boundary cells as a multipotent and self-renewing Sox2(+) cell population. Inhibition of Sox2 in boundaries led to enhanced and aberrant neural differentiation together with inhibition in cell-proliferation, whereas Sox2 mis-expression attenuated neurogenesis, confirming its significant function in hindbrain neuronal organization.
conclusionsData obtained in this study deciphers a novel role of hindbrain boundaries as repetitive pools of neural stem/progenitor cells, which provide proliferating progenitors and differentiating neurons in a Sox2-dependent regulation.
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