ArticlePloS one2016
Evaluation of Reference Genes for Reverse Transcription Quantitative PCR Studies of Physiological Responses in the Ghost Moth, Thitarodes armoricanus (Lepidoptera, Hepialidae).
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed.
- Stability Evaluation of Reference Genes inInsects · 2025Article
- Article
- Reference gene identification and evaluation for accurate qPCR normalization in Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae).Molecular biology reports · 2025Article
- Evaluation of stable reference genes for RT-qPCR analysis in Antheraea assamensis (Lepidoptera: Saturniidae) during pathogenic infections.Molecular biology reports · 2025Article
- Assessment of housekeeping genes stability for gene transcription regulation analysis of Spodoptera littoralis (Lepidoptera: Noctuidae) under Spodoptera littoralis nucleopolyhedrovirus viral infection.Molecular biology reports · 2024Article
- Selection and validation of optimal reference genes for RT-qPCR analyses inFrontiers in physiology · 2023Article
- Identifying optimal reference genes for gene expression studies in Eurasian spruce bark beetle, Ips typographus (Coleoptera: Curculionidae: Scolytinae).Scientific reports · 2022Article
- Reference Genes for Expression Analyses by qRT-PCR inInsects · 2022Article
- Article
- Selection and validation of reference genes for RT-qPCR-based analyses ofFrontiers in physiology · 2022Article
- Article
- Selection and Validation of Reference Genes for RT-qPCR Normalization inFrontiers in physiology · 2021Article
- Article
- Identification and Evaluation of Reference Genes for Normalization of Gene Expression in Developmental Stages, Sexes, and Tissues of Diaphania caesalis (Lepidoptera, Pyralidae).Journal of insect science (Online) · 2020Article
- Identification and validation of potential reference gene for effective dsRNA knockdown analysis in Chilo partellus.Scientific reports · 2019Article
- Evaluation and Validation of Reference Genes for Quantitative Real-Time PCR in Helopeltis theivora Waterhouse (Hemiptera: Miridae).Scientific reports · 2019Article
- Evaluation of Reference Genes for Real-Time Quantitative PCR Analysis in Larvae ofFrontiers in physiology · 2018Article
- Selection of Reference Genes for the Normalization of RT-qPCR Data in Gene Expression Studies in Insects: A Systematic Review.Frontiers in physiology · 2018Article
- Scratching Stimuli of Mycelia Influence Fruiting Body Production and ROS-Scavenging Gene Expression ofMycobiology · 2018Article
- Selection of housekeeping genes and demonstration of RNAi in cotton leafhopper, Amrasca biguttula biguttula (Ishida).PloS one · 2018Article
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Abstract
Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is the sensitive method to quantify the expression levels of target genes on the basis of endogenous control. An appropriate reference gene set for normalization is essential for reliable results. The ghost moth, Thitarodes armoricanus, a host species of a medicinal fungus, Ophiocordyceps sinensis, is an economically important member of the Lepidoptera. Recent studies have focused on the mechanism of adaptation of this species to its high-altitude environment and host immune response to O. sinensis infection and RT-qPCR is commonly used in these studies to decipher the genetic basis of physiological functions. However, a thorough assessment of candidate reference genes in the genus Thitarodes is lacking. Here, the expression levels of eight candidate reference genes (ACT, EF, EIF4A, GAPDH, G6PDH, RPL13A, TUB and 18S) in T. armoricanus at different developmental stages and in different body parts of the seventh instar larvae were analyzed, along with larvae kept under low temperatures, larvae exposed to two fungal infections and larvae fed different diets. Three established software programs-Bestkeeper, geNorm and NormFinder-were employed to calculate variation among the treatments. The results revealed that the best-suited reference genes differed across the treatments, with EF, EIF4A and GAPDH found to be the best suited for the different developmental stages and larvae body parts; EF, EIF4A and RPL13A found to be the best suited for low-temperature challenge; and EF, EIF4A and TUB found to be the best suited for the fungal infections and dietary treatments. This study thus further contributes to the establishment of an accurate method for normalizing RT-qPCR results for T. armoricanus and serves as a reference for gene expression studies of related insect species.
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