ArticlePloS one2016
Effects of Chondroitinase ABC-Mediated Proteoglycan Digestion on Decellularization and Recellularization of Articular Cartilage.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 46 citations in OpenAlex.
- A disease-severity-responsive nanoparticle enables potent ghrelin messenger RNA therapy in osteoarthritis.Nature nanotechnology · 2026Article
- Bioassembly of Region-Specific Fibrocartilage Microtissues to Engineer Zonally Defined Meniscal Grafts.Advanced healthcare materials · 2025Article
- The introduction and evaluation of novel decellularized extracellular matrix/gellan gum bioprinting scaffolds for cartilage tissue engineering.Scientific reports · 2025Article
- A Tissue-Engineered Construct Based on a Decellularized Scaffold and the Islets of Langerhans: A Streptozotocin-Induced Diabetic Model.Life (Basel, Switzerland) · 2024Article
- Decellularized extracellular matrix biomaterials for regenerative therapies: Advances, challenges and clinical prospects.Bioactive materials · 2024Review
- Nerve regeneration using decellularized tissues: challenges and opportunities.Frontiers in neuroscience · 2023Review
- ECM roles and biomechanics in cardiac tissue decellularization.American journal of physiology. Heart and circulatory physiology · 2022Review
- Material Characterisation and Stratification of Conjunctival Epithelial Cells on Electrospun Poly(ε-Caprolactone) Fibres Loaded with Decellularised Tissue Matrices.Pharmaceutics · 2021Article
- Applications of Biocompatible Scaffold Materials in Stem Cell-Based Cartilage Tissue Engineering.Frontiers in bioengineering and biotechnology · 2021Review
- Tissue-Specific Decellularization Methods: Rationale and Strategies to Achieve Regenerative Compounds.International journal of molecular sciences · 2020Review
- 3D Bioprinting: from Benches to Translational Applications.Small (Weinheim an der Bergstrasse, Germany) · 2019Review
- Applications of decellularized extracellular matrix in bone and cartilage tissue engineering.Bioengineering & translational medicine · 2019Review
- Current Concepts in Meniscus Tissue Engineering and Repair.Advanced healthcare materials · 2018Review
- Glycosaminoglycans contribute to extracellular matrix fiber recruitment and arterial wall mechanics.Biomechanics and modeling in mechanobiology · 2017Article
- The Challenge in Using Mesenchymal Stromal Cells for Recellularization of Decellularized Cartilage.Stem cell reviews and reports · 2017Review
- Article
- Decellularization for the retention of tissue niches.Journal of tissue engineeringReview
- Post-decellularization techniques ameliorate cartilage decellularization process for tissue engineering applications.Journal of tissue engineeringReview
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Articular cartilage has a limited capacity to heal itself and thus focal defects often result in the development of osteoarthritis. Current cartilage tissue engineering strategies seek to regenerate injured tissue by creating scaffolds that aim to mimic the unique structure and composition of native articular cartilage. Decellularization is a novel strategy that aims to preserve the bioactive factors and 3D biophysical environment of the native extracellular matrix while removing potentially immunogenic factors. The purpose of this study was to develop a procedure that can enable decellularization and recellularization of intact articular cartilage matrix. Full-thickness porcine articular cartilage plugs were decellularized with a series of freeze-thaw cycles and 0.1% (w/v) sodium dodecyl sulfate detergent cycles. Chondroitinase ABC (ChABC) was applied before the detergent cycles to digest glycosaminoglycans in order to enhance donor chondrocyte removal and seeded cell migration. Porcine synovium-derived mesenchymal stem cells were seeded onto the decellularized cartilage scaffolds and cultured for up to 28 days. The optimized decellularization protocol removed 94% of native DNA per sample wet weight, while collagen content and alignment were preserved. Glycosaminoglycan depletion prior to the detergent cycles increased removal of nuclear material. Seeded cells infiltrated up to 100 μm into the cartilage deep zone after 28 days in culture. ChABC treatment enhances decellularization of the relatively dense, impermeable articular cartilage by reducing glycosaminoglycan content. ChABC treatment did not appear to affect cell migration during recellularization under static, in vitro culture, highlighting the need for more dynamic seeding methods.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.