ArticlePloS one2016
RuBisCO in Non-Photosynthetic Alga Euglena longa: Divergent Features, Transcriptomic Analysis and Regulation of Complex Formation.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- A cryptic plastid and a novel mitochondrial plasmid inOpen biology · 2024Article
- Complex Endosymbioses I: From Primary to Complex Plastids, Serial Endosymbiotic Events.Methods in molecular biology (Clifton, N.J.) · 2024Article
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- Catalase impairsVirulence · 2021Article
- The Cryptic Plastid ofmSphere · 2020Article
- Catalase and Ascorbate Peroxidase in Euglenozoan Protists.Pathogens (Basel, Switzerland) · 2020Article
- Comparative Plastid Genomics of Cryptomonas Species Reveals Fine-Scale Genomic Responses to Loss of Photosynthesis.Genome biology and evolution · 2020Article
- Highly Reduced Plastid Genomes of the Non-photosynthetic DictyochophyceansFrontiers in plant science · 2020Article
- Principles of plastid reductive evolution illuminated by nonphotosynthetic chrysophytes.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Peculiar features of the plastids of the colourless alga Euglena longa and photosynthetic euglenophytes unveiled by transcriptome analyses.Scientific reports · 2018Article
- Reductive evolution of chloroplasts in non-photosynthetic plants, algae and protists.Current genetics · 2018Review
- Extensive molecular tinkering in the evolution of the membrane attachment mode of the Rheb GTPase.Scientific reports · 2018Article
- An intact plastid genome is essential for the survival of colorless Euglena longa but not Euglena gracilis.Current genetics · 2017Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Euglena longa, a close relative of the photosynthetic model alga Euglena gracilis, possesses an enigmatic non-photosynthetic plastid. Its genome has retained a gene for the large subunit of the enzyme RuBisCO (rbcL). Here we provide new data illuminating the putative role of RuBisCO in E. longa. We demonstrated that the E. longa RBCL protein sequence is extremely divergent compared to its homologs from the photosynthetic relatives, suggesting a possible functional shift upon the loss of photosynthesis. Similarly to E. gracilis, E. longa harbors a nuclear gene encoding the small subunit of RuBisCO (RBCS) as a precursor polyprotein comprising multiple RBCS repeats, but one of them is highly divergent. Both RBCL and the RBCS proteins are synthesized in E. longa, but their abundance is very low compared to E. gracilis. No RBCS monomers could be detected in E. longa, suggesting that processing of the precursor polyprotein is inefficient in this species. The abundance of RBCS is regulated post-transcriptionally. Indeed, blocking the cytoplasmic translation by cycloheximide has no immediate effect on the RBCS stability in photosynthetically grown E. gracilis, but in E. longa, the protein is rapidly degraded. Altogether, our results revealed signatures of evolutionary degradation (becoming defunct) of RuBisCO in E. longa and suggest that its biological role in this species may be rather unorthodox, if any.
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