ArticlePloS one2016
Introgression of opaque2 into Waxy Maize Causes Extensive Biochemical and Proteomic Changes in Endosperm.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- Regulatory Mechanisms of Free Umami Amino Acid Accumulation in Fresh Waxy Kernels: Insights from Transcriptome and Metabolomics Analyses.Foods (Basel, Switzerland) · 2025Article
- High amylopectin in waxy maize synergistically affects seed germination and seedling vigour over traditional maize genotypes.Journal of applied genetics · 2025Article
- Unraveling the interactive effect of opaque2 and waxy1 genes on kernel nutritional qualities and physical properties in maize (Zea mays L.).Scientific reports · 2025Article
- Comparative transcriptome profiling reveals the mechanism of increasing lysine and tryptophan content through pyramidingBreeding science · 2024Article
- Nitrogen fertilizer application rate affects the dynamic metabolism of nitrogen and carbohydrates in kernels of waxy maize.Frontiers in plant science · 2024Article
- Variations in Total Protein and Amino Acids in the Sequenced Sorghum Mutant Library.Plants (Basel, Switzerland) · 2023Article
- Using the dominant mutation geneMolecular breeding : new strategies in plant improvement · 2022Article
- Pollen staining is a rapid and cost-effective alternative to marker-assisted selection for recessivePhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Development and validation of rapid and cost-effective protocol for estimation of amylose and amylopectin in maize kernels.3 Biotech · 2022Article
- Analysis of the genetic architecture of maize kernel size traits by combined linkage and association mapping.Plant biotechnology journal · 2020Article
- The Zea mays mutants opaque2 and opaque16 disclose lysine change in waxy maize as revealed by RNA-Seq.Scientific reports · 2019Article
- Article
- Molecular Mechanisms Underlying Increase in Lysine Content of Waxy Maize through the Introgression of theInternational journal of molecular sciences · 2019Article
- Molecular analysis of mutant3 Biotech · 2019Article
- Microsatellite marker-based characterization of waxy maize inbreds for their utilization in hybrid breeding.3 Biotech · 2017Article
- Analysis of the genetic architecture of maize ear and grain morphological traits by combined linkage and association mapping.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2017Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
13 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Waxy maize is prevalently grown in China and other countries due to the excellent characters and economic value. However, its low content of lysine can't meet the nutritional requirements of humans and livestock. In the present study, we introgressed the opaque2 (o2) allele into waxy maize line Zhao OP-6/O2O2 by using marker-assisted selection (MAS) technique and successfully improved the lysine content and quality of waxy maize. Transcript abundance analysis indicated that the wx1 expression levels had no difference between Zhao OP-6/o2o2 and Zhao OP-6/O2O2. However, Zhao OP-6/o2o2 was characterized by a phenotype of hard and vitreous kernels and accumulation of protein bodies at smaller size (one third of that of parents) but in larger numbers. Biochemical analyses showed that Zhao OP-6/o2o2 had 16.7% less free amino acids than Zhao OP-6/O2O2, especially those derived from glycolytic intermediates, but its content of lysine was increased by 51.6% (0.47% vs. 0.31%). The content of amylopectin was 98.5% in Zhao OP-6/o2o2, significantly higher than that in Zhao OP-6/O2O2 (97.7%). Proteomic analyses indicated that o2 introgression not only decreased the accumulation of various zein proteins except for 27-kDa γ-zein, but also affected other endosperm proteins related to amino acid biosynthesis, starch-protein balance, stress response and signal transduction. This study gives us an intriguing insight into the metabolism changes in endosperm of waxy maize introgressed with opaque2.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.