ArticlePloS one2016
Oxidation of Са2+-Binding Domain of NADPH Oxidase 5 (NOX5): Toward Understanding the Mechanism of Inactivation of NOX5 by ROS.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- F-actin-dependent activation of NADPH oxidase 5 by the vacuolar-type ATPase.The Journal of biological chemistry · 2026Article
- Zinc binding to a conserved motif in STIM1 induces clustering and SOCE activation.Cell communication and signaling : CCS · 2025Article
- NADPH oxidases: redox regulation of cell homeostasis and disease.Physiological reviews · 2025Review
- NADPH Oxidases: From Molecular Mechanisms to Current Inhibitors.Journal of medicinal chemistry · 2023Review
- Mechanism of ZnBiomolecules · 2021Article
- Nitric oxide and heme-NO stimulate superoxide production by NADPH oxidase 5.Free radical biology & medicine · 2021Article
- An AI-Powered Blood Test to Detect Cancer Using NanoDSF.Cancers · 2021Article
- Interrelation between ROS and CaRedox biology · 2020Review
- Hypoxia-Inducible Factor-1: A Potential Target to Treat Acute Lung Injury.Oxidative medicine and cellular longevity · 2020Review
- Evaluation of the NOX5 protein expression and oxidative stress in sperm from asthenozoospermic men compared to normozoospermic men.Journal of endocrinological investigation · 2019Article
- Pharmacological characterization of the seven human NOX isoforms and their inhibitors.Redox biology · 2019Article
- NOX5: Molecular biology and pathophysiology.Experimental physiology · 2019Review
- Article
- Reactive oxygen species and male reproductive hormones.Reproductive biology and endocrinology : RB&E · 2018Review
- Differential scanning calorimetry of plasma in glioblastoma: toward a new prognostic / monitoring tool.Oncotarget · 2018Article
- NADPH oxidase 5 (NOX5)-induced reactive oxygen signaling modulates normoxic HIF-1α and p27Molecular carcinogenesis · 2017Article
- Zinc binding to RNA recognition motif of TDP-43 induces the formation of amyloid-like aggregates.Scientific reports · 2017Article
- The Impact of NADPH Oxidase 5 Activity and Reactive Oxygen Species on Capacitated Human Sperm.Journal of reproduction & infertilityArticle
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
NOX5 protein, one of the most active generators of reactive oxygen species (ROS), plays an important role in many processes, including regulation of cell growth, death and differentiation. Because of its central role in ROS generation, it needs to be tightly regulated to guarantee cellular homeostasis. Contrary to other members of NADPH-oxidases family, NOX5 has its own regulatory calcium-binding domain and thus could be activated directly by calcium ions. While several mechanisms of activation have been described, very little is known about the mechanisms that could prevent the overproduction of ROS by NOX5. In the present study using calorimetric methods and circular dichroism we found that oxidation of cysteine and methionine residues of NOX5 decreases binding of Ca2+ ions and perturbs both secondary and tertiary structure of protein. Our data strongly suggest that oxidation of calcium-binding domain of NOX5 could be implicated in its inactivation, serving as a possible defense mechanism against oxidative stress.
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