ArticleOncotarget2016
Optogenetic regulation of site-specific subtelomeric DNA methylation.
Article in Oncotarget, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Editing DNA methylation in vivo.Nature communications · 2025Article
- Light-Activation of DNA-Methyltransferases.Angewandte Chemie (International ed. in English) · 2021Article
- High-altitude adaptation: Role of genetic and epigenetic factors.Journal of biosciences · 2021Review
- Article
- A Photo-responsive Small-Molecule Approach for the Opto-epigenetic Modulation of DNA Methylation.Angewandte Chemie (International ed. in English) · 2019Article
- Metals and Mechanisms of Carcinogenesis.Annual review of pharmacology and toxicology · 2019Review
- The methylation and telomere landscape in two families of marsupials with different rates of chromosome evolution.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2018Article
- Cell Type and Species-specific Patterns in Neuronal and Non-neuronal Methylomes of Human and Chimpanzee Cortices.Cerebral cortex (New York, N.Y. : 1991) · 2018Article
- Epigenetic features of human telomeres.Nucleic acids research · 2018Article
- The Alteration of Subtelomeric DNA Methylation in Aging-Related Diseases.Frontiers in genetics · 2018Review
- Novel Epigenetic Techniques Provided by the CRISPR/Cas9 System.Stem cells international · 2018Review
- Epigenetic Editing of Ascl1 Gene in Neural Stem Cells by Optogenetics.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Telomere length homeostasis, critical for chromosomal integrity and genome stability, is controlled by intricate molecular regulatory machinery that includes epigenetic modifications. Here, we examine site-specific and spatiotemporal alteration of the subtelomeric methylation of CpG islands using optogenetic tools to understand the epigenetic regulatory mechanisms of telomere length maintenance. Human DNA methyltransferase3A (DNMT3A) were assembled selectively at chromosome ends by fusion to cryptochrome 2 protein (CRY2) and its interacting complement, the basic helix loop helix protein-1 (CIB1). CIB1 was fused to the telomere-associated protein telomere repeat binding factor-1 (TRF1), which localized the protein complex DNMT3A-CRY2 at telomeric regions upon excitation by blue-light monitored by single-molecule fluorescence analyses. Increased methylation was achieved selectively at subtelomeric CpG sites on the six examined chromosome ends specifically after blue-light activation, which resulted in progressive increase in telomere length over three generations of HeLa cell replications. The modular design of the fusion constructs presented here allows for the selective substitution of other chromatin modifying enzymes and for loci-specific targeting to regulate the epigenetic pathways at telomeres and other selected genomic regions of interest.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.