Evidence map›Paper›PMID 27390760›Full record

ArticleBio-protocol

X-gal Staining on Adult Mouse Brain Sections.

Hiroshi Kokubu, Janghoo Lim

Open access · greenAbstract read
In one paragraph

Article in Bio-protocol. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact, top 78% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 14 citations in OpenAlex.

  1. MidbrainbioRxiv : the preprint server for biology · 2026
    Article
  2. Article
  3. Retinoic acid receptor assembly dynamics governs dual functions in cochlear organogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Hiroshi KokubuDepartment of Genetics; Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, USA.
Janghoo LimDepartment of Genetics; Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, USA.
Yale University · US

Funding

The role of Nemo-like kinase in neurodegenerationR00NS064146 · NINDS · YALE UNIVERSITY · PI LIM, JANGHOO · 2010 to 2012
$721k
The role of Nemo-like kinase in neurodegenerationK99NS064146 · NINDS · BAYLOR COLLEGE OF MEDICINE · PI LIM, JANGHOO · 2009 to 2010
$175k
NINDS NIH HHS K99 NS064146NINDS NIH HHS R00 NS064146
6 · The paper itself

Abstract

Knowing expression patterns of given proteins is very important to understand their functions. Immunostaining analysis with specific antibodies is commonly used to identify cells or tissues expressing proteins of interest. Although this technique is regularly used, it requires high quality of specific antibodies and there is no good quality of antibody available for certain proteins. Alternatively, X-gal staining is also used to analyze protein expression pattern. It is simple and routinely used to detect expression pattern of any proteins of interest

Identifiers

PMID27390760
PMCPMC4932900
OpenAlexW2515946190

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.