ArticleHorticulture research2016
Knockdown of MLO genes reduces susceptibility to powdery mildew in grapevine.
Article in Horticulture research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 191 citations in OpenAlex.
- Chemical Priming by Neonicotinoids Unveils CaNEN4 as a Susceptibility Gene Against Phytophthora capsici in Pepper.Molecular plant pathology · 2026Article
- CRISPR-Cas9-Mediated Knockout ofPlants (Basel, Switzerland) · 2025Article
- Review
- Advances in grape and pathogen genomics toward durable grapevine disease resistance.Journal of experimental botany · 2025Review
- Transcriptome profiling of symptomatic vs. asymptomatic grapevine plants reveals candidate genes for plant improvement against trunk diseases.BMC plant biology · 2025Article
- Silencing of disease susceptibility genes: an effective disease resistance strategy against fungal pathogens.Plant cell reports · 2025Review
- CRISPR/Cas9-driven double modification of grapevine MLO6-7 imparts powdery mildew resistance, while editing of NPR3 augments powdery and downy mildew tolerance.The Plant journal : for cell and molecular biology · 2025Article
- Advances in the molecular mechanism of grapevine resistance to fungal diseases.Molecular horticulture · 2025Review
- The Hansen's baccata #2 gene Rvi12_Cd5 confers scab resistance to the susceptible apple cultivar "Gala Galaxy".The Plant journal : for cell and molecular biology · 2025Article
- A New Approach for CRISPR/Cas9 Editing and Selection of Pathogen-Resistant Plant Cells of Wine Grape cv. 'Merlot'.International journal of molecular sciences · 2024Article
- CsMLO8/11 are required for full susceptibility of cucumber stem to powdery mildew and interact with CsCRK2 and CsRbohD.Horticulture research · 2024Article
- Novel loci associated with resistance to downy and powdery mildew in grapevine.Frontiers in plant science · 2024Article
- Enhancing powdery mildew resistance in soybean by targeted mutation of MLO genes using the CRISPR/Cas9 system.BMC plant biology · 2023Article
- Tae-miR397 Negatively Regulates Wheat Resistance toPlants (Basel, Switzerland) · 2023Article
- Integrated Molecular and Bioinformatics Approaches for Disease-Related Genes in Plants.Plants (Basel, Switzerland) · 2023Review
- Genome engineering of disease susceptibility genes for enhancing resistance in plants.Functional & integrative genomics · 2023Review
- The Role of Italy in the Use of Advanced Plant Genomic Techniques on Fruit Trees: State of the Art and Future Perspectives.International journal of molecular sciences · 2023Review
- Inactivation of tomatoFrontiers in plant science · 2023Article
- Genome-wide identification, evolution, and expression analysis ofFrontiers in plant science · 2023Article
- Review
5 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Erysiphe necator is the causal agent of powdery mildew (PM), one of the most destructive diseases of grapevine. PM is controlled by sulfur-based and synthetic fungicides, which every year are dispersed into the environment. This is why PM-resistant varieties should become a priority for sustainable grapevine and wine production. PM resistance can be achieved in other crops by knocking out susceptibility S-genes, such as those residing at genetic loci known as MLO (Mildew Locus O). All MLO S-genes of dicots belong to the phylogenetic clade V, including grapevine genes VvMLO7, 11 and 13, which are upregulated during PM infection, and VvMLO6, which is not upregulated. Before adopting a gene-editing approach to knockout candidate S-genes, the evidence that loss of function of MLO genes can reduce PM susceptibility is necessary. This paper reports the knockdown through RNA interference of VvMLO6, 7, 11 and 13. The knockdown of VvMLO6, 11 and 13 did not decrease PM severity, whereas the knockdown of VvMLO7 in combination with VvMLO6 and VvMLO11 reduced PM severity up to 77%. The knockdown of VvMLO7 and VvMLO6 seemed to be important for PM resistance, whereas a role for VvMLO11 does not seem likely. Cell wall appositions (papillae) were present in both resistant and susceptible lines in response to PM attack. Thirteen genes involved in defense were less upregulated in infected mlo plants, highlighting the early mlo-dependent disruption of PM invasion.
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Registered trials
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