ArticleScientific reports2016
A novel single cell method to identify the genetic composition at a single nuclear body.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- Identification of Genomic Loci Responsible for the Formation of Nuclear Domains Using Lampbrush Chromosomes.Non-coding RNA · 2019Review
- PML nuclear bodies contribute to the basal expression of the mTOR inhibitor DDIT4.Scientific reports · 2017Article
- Light behind the curtain: photoregulation of nuclear architecture and chromatin dynamics in plants.The New phytologist · 2016Article
- Gene locations may contribute to predicting gene regulatory relationships.Journal of Zhejiang University. Science. BArticle
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Authors and funding
5 authors.
Funding
Abstract
Gene loci make specific associations with compartments of the nucleus (e.g. the nuclear envelope, nucleolus, and transcription factories) and this association may determine or reflect a mechanism of genetic control. With current methods, it is not possible to identify sets of genes that converge to form a "gene hub" as there is a reliance on loci-specific probes, or immunoprecipitation of a particular protein from bulk cells. We introduce a method that will allow for the identification of loci contained within the vicinity of a single nuclear body in a single cell. For the first time, we demonstrate that the DNA sequences originating from a single sub-nuclear structure in a single cell targeted by two-photon irradiation can be determined, and mapped to a particular locus. Its application to single PML nuclear bodies reveals ontologically related loci that frequently associate with each other and with PML bodies in a population of cells, and a possible nuclear body targeting role for specific transcription factor binding sites.
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Registered trials
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