ArticleEnvironmental toxicology and pharmacology2016
The application of the comet assay to assess the genotoxicity of environmental pollutants in the nematode Caenorhabditis elegans.
Article in Environmental toxicology and pharmacology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 42 citations in OpenAlex.
- A Review of the Literature on the Endocrine Disruptor Activity Testing of Bisphenols inJournal of xenobiotics · 2026Review
- DNA repair, recombination, and damage signaling.Genetics · 2022Review
- A fast and reliable method for monitoring genomic instability in the model organism Caenorhabditis elegans.Archives of toxicology · 2021Article
- Benzo[a]pyrene and Caenorhabditis elegans: defining the genotoxic potential in an organism lacking the classical CYP1A1 pathway.Archives of toxicology · 2021Article
- Molecular and cellular responses to short exposure to bisphenols A, F, and S and eluates of microplastics in C. elegans.Environmental science and pollution research international · 2021Article
- Article
- Measurement of Oxidatively Induced DNA Damage inAnalytical chemistry · 2019Article
- DNA damage and physiological responses in an Indian major carp Labeo rohita exposed to an antimicrobial agent triclosan.Fish physiology and biochemistry · 2019Article
- Neuronal XBP-1 Activates Intestinal Lysosomes to Improve Proteostasis in C. elegans.Current biology : CB · 2019Article
- Co-exposure to polystyrene plastic beads and polycyclic aromatic hydrocarbon contaminants in fish gill (RTgill-W1) and intestinal (RTgutGC) epithelial cells derived from rainbow trout (Oncorhynchus mykiss).Environmental pollution (Barking, Essex : 1987) · 2019Article
- Drosophila melanogaster as model organism for monitoring and analyzing genotoxicity associated with city air pollution.Environmental science and pollution research international · 2018Article
- High-glucose toxicity is mediated by AICAR-transformylase/IMP cyclohydrolase and mitigated by AMP-activated protein kinase inThe Journal of biological chemistry · 2018Article
- Counting Caenorhabditis elegans: Protocol Optimization and Applications for Population Growth and Toxicity Studies in Liquid Medium.Scientific reports · 2018Article
- Measuring the Effect of Chemicals on the Growth and Reproduction of Caenorhabditis elegans.Journal of visualized experiments : JoVE · 2017Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
This study aimed to establish a protocol for cell dissociation from the nematode Caenorhabditis elegans (C. elegans) to assess the genotoxicity of the environmental pollutant benzo[a]pyrene (BaP) using the alkaline version of the single cell electrophoresis assay (comet assay). BaP genotoxicity was assessed in C. elegans (wild-type [WT]; N2, Bristol) after 48h exposure (0-40μM). Induction of comets by BaP was concentration-dependent up to 20μM; comet% tail DNA was ∼30% at 20μM BaP and ∼10% in controls. Similarly, BaP-induced DNA damage was evaluated in C. elegans mutant strains deficient in DNA repair. In xpa-1 and apn-1 mutants BaP-induced comet formation was diminished to WT background levels suggesting that the damage formed by BaP that is detected in the comet assay is not recognised in cells deficient in nucleotide and base excision repair, respectively. In summary, our study provides a protocol to evaluate DNA damage of environmental pollutants in whole nematodes using the comet assay.
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