Evidence map›Paper›PMID 27389619›Full record

ArticleScientific reports2016

Construction of high-density genetic linkage map and identification of flowering-time QTLs in orchardgrass using SSRs and SLAF-seq.

Xinxin Zhao, Linkai Huang, Xinquan Zhang, Jianping Wang, Defei Yan, Ji Li, Lu Tang, Xiaolong Li, Tongwei Shi

Open access · goldAbstract readComparative Study
In one paragraph

Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
5.4field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 70 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Xinxin ZhaoDepartment of Grassland Science, Sichuan Agricultural University, Chengdu, 611130, China.
Linkai HuangDepartment of Grassland Science, Sichuan Agricultural University, Chengdu, 611130, China.
Xinquan ZhangDepartment of Grassland Science, Sichuan Agricultural University, Chengdu, 611130, China.
Jianping WangAgronomy Department, University of Florida, FL, 32610, USA.
Defei YanDepartment of Grassland Science, Sichuan Agricultural University, Chengdu, 611130, China.
Ji LiDepartment of Grassland Science, Sichuan Agricultural University, Chengdu, 611130, China.
Lu TangDepartment of Grassland Science, Sichuan Agricultural University, Chengdu, 611130, China.
Xiaolong LiBiomarker Technologies Corporation, Beijing, 101300, China.
Tongwei ShiBiomarker Technologies Corporation, Beijing, 101300, China.
Sichuan Agricultural University · CNUniversity of Florida · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Orchardgrass (Dactylis glomerata L.) is one of the most economically important perennial, cool-season forage species grown and pastured worldwide. High-density genetic linkage mapping is a valuable and effective method for exploring complex quantitative traits. In this study, we developed 447,177 markers based on SLAF-seq and used them to perform a comparative genomics analysis. Perennial ryegrass sequences were the most similar (5.02%) to orchardgrass sequences. A high-density linkage map of orchardgrass was constructed using 2,467 SLAF markers and 43 SSRs, which were distributed on seven linkage groups spanning 715.77 cM. The average distance between adjacent markers was 0.37 cM. Based on phenotyping in four environments, 11 potentially significant quantitative trait loci (QTLs) for two target traits-heading date (HD) and flowering time (FT)-were identified and positioned on linkage groups LG1, LG3, and LG5. Significant QTLs explained 8.20-27.00% of the total phenotypic variation, with the LOD ranging from 3.85-12.21. Marker167780 and Marker139469 were associated with FT and HD at the same location (Ya'an) over two different years. The utility of SLAF markers for rapid generation of genetic maps and QTL analysis has been demonstrated for heading date and flowering time in a global forage grass.

Indexed as

Quantitative Trait LociChromosome MappingDactylisFlowersGenetic LinkageGenetic MarkersMicrosatellite RepeatsPhenotypePlant ProteinsSeasonsSequence Analysis, DNAGenetic MarkersPlant Proteins

Identifiers

PMID27389619
PMCPMC4937404
OpenAlexW2472977044

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.