ArticleJournal of artificial organs : the official journal of the Japanese Society for Artificial Organs2016
Platelet-rich plasma stimulates human dermal fibroblast proliferation via a Ras-dependent extracellular signal-regulated kinase 1/2 pathway.
Article in Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- mRNA Therapeutics for Skin Rejuvenation: From Aging Atlases to Clinical Translation.Experimental dermatology · 2026Review
- Intraoperative Autologous Adipose-Derived Therapies and PRP as Add-On in the Surgical Treatment of Cryptoglandular and Crohn's Disease-Related Perianal Fistula-A Systematic Review.Bioengineering (Basel, Switzerland) · 2026Review
- Transforming PRP into a shelf-stable therapy: Lyophilization preserves angiogenic and regenerative properties.Iranian journal of basic medical sciences · 2026Article
- The Combination of Stromal Vascular Fraction Cells and Platelet-Rich Plasma Increases Fibroblast Growth Factor 2 and Insulin-Like Growth Factor 1 in Full-Thickness Burns in Animal Model.Annals of burns and fire disasters · 2024Article
- Article
- The effect of allogenic freeze-dried platelet-rich plasma in increasing the number of fibroblasts and neovascularization in wound healing.Annals of medicine and surgery (2012) · 2022Article
- Role of MSC-derived small extracellular vesicles in tissue repair and regeneration.Frontiers in cell and developmental biology · 2022Review
- Comparison of the efficacy of cryopreserved human platelet lysate and refrigerated lyophilized human platelet lysate for wound healing.Regenerative therapy · 2019Article
- Platelet-rich plasma enhances the proliferation of human adipose stem cells through multiple signaling pathways.Stem cell research & therapy · 2018Article
- Journal of Artificial Organs 2016: the year in review : Journal of Artificial Organs Editorial Committee.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2017Review
- Post-decellularization techniques ameliorate cartilage decellularization process for tissue engineering applications.Journal of tissue engineeringReview
Corrections and comments
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Authors and funding
6 authors.
Funding
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Abstract
Platelet-rich plasma (PRP) contains a high concentration of several growth factors and contributes to soft-tissue engineering and wound healing. However, the effect of PRP on human dermal fibroblast proliferation and responses is unknown. This was investigated in the present study using PRP prepared from the whole human blood using the double-spin method. Human dermal fibroblast cultures were established from skin samples collected during plastic surgery. Platelet concentration and growth factor levels in PRP were estimated, and a cell proliferation assay was carried out after PRP treatment. The role of Ras-dependent extracellular signal-regulated kinase (ERK)1/2 in the effects of PRP was investigated in human dermal fibroblasts by suppressing ERK1/2 expression with an inhibitor or by short interfering (si)RNA-mediated knockdown, and assessing ERK1/2 phosphorylation by western blotting as well as proliferation in PRP-treated cells. We found that PRP stimulated human dermal fibroblast proliferation, which was suppressed by ERK1/2 inhibitor treatment (P < 0.01). ERK1/2 phosphorylation was increased in the presence of PRP, while siRNA-mediated knockdown of ERK1/2 blocked cell proliferation normally induced by PRP treatment (P < 0.01). These results demonstrate that PRP induces human dermal fibroblast proliferation via activation of ERK1/2 signaling. Our findings provide a basis for the development of agents that can promote wound healing and can be applied to soft-tissue engineering.
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Registered trials
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