ArticleNature protocols2016
Laboratory breeding of the short-lived annual killifish Nothobranchius furzeri.
Article in Nature protocols, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed.
- A lifespan atlas of the killifish testis defines a pronounced mid-age remodeling shift during testicular aging.BMC biology · 2026Article
- Sexual selection and reproductive success in mesocosm populations of African annual killifish.Journal of fish biology · 2026Article
- Developing species-specific welfare scoresheets for the African Turquoise Killifish.Scientific reports · 2026Article
- First attempt at using a synthetic diet in short-lived killifish, a vertebrate model of ageing.Journal of fish biology · 2026Article
- Contributions of aquatic organisms to understanding the vertebrate ocular lens.Frontiers in ophthalmology · 2026Review
- Sex-specific effects of automated feeding on lifespan and reproduction inFrontiers in aging · 2026Article
- Senescent-associated β-galactosidase accumulation does not correlate with lifespan in different strains ofmicroPublication biology · 2026Article
- Alternative social and reproductive niches linked to intra-sexual color variation in a facultatively protogynous North American annual killifish, Millerichthys robustus.Scientific reports · 2025Article
- Fins as a reliable surrogate tissue for age-related changes of telomeres and DNA methylation in gonads of a short-lived fish.Biogerontology · 2025Article
- Early-life growth and cellular heterogeneity in the short-lived African turquoise killifish telencephalon.Biology open · 2025Article
- A short-lived fish with long-lasting effects: hallmarks of aging inFrontiers in aging · 2025Review
- Single-cell sequencing unveils the impact of aging on the progenitor cell diversity in the telencephalon of the female killifish N. furzeri.Aging cell · 2024Article
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- Axis formation in annual killifish: Nodal and β-catenin regulate morphogenesis without Huluwa prepatterning.Science (New York, N.Y.) · 2024Article
- Refeeding-associated AMPKNature aging · 2023Article
- Zebrafish as an Emerging Model for Sarcopenia: Considerations, Current Insights, and Future Directions.International journal of molecular sciences · 2023Review
- A short dasatinib and quercetin treatment is sufficient to reinstate potent adult neuroregenesis in the aged killifish.NPJ Regenerative medicine · 2023Article
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- Impaired fin regeneration and angiogenesis in aged zebrafish and turquoise killifish.Biology open · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Turquoise killifish, Nothobranchius furzeri, have an intrinsically short life span, with a median life span of <6 months and a maximum (90%) life span of 9 months. This short life span, which is unique among vertebrates, evolved naturally and has resulted in N. furzeri becoming a widely used laboratory model species in aging research and other disciplines. Here, we describe a protocol for the maintenance and breeding of the species under laboratory conditions. We provide details for egg incubation, hatching, everyday care of juvenile and adult fish, breeding and treatment of most common diseases. Emphasis is given to the fact that the requirements of N. furzeri substantially differ from those of other fish model taxa; N. furzeri live brief lives and in nature undergo nonaquatic embryo development, with consequences for their laboratory culture.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.