ArticlePloS one2016
FRAP to Characterize Molecular Diffusion and Interaction in Various Membrane Environments.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 116 citations in OpenAlex.
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- Model Lipid Bilayers as Platforms for Replicating Bacterial Membranes: A Study of Dynamics and Structural Heterogeneity.The journal of physical chemistry. B · 2026Article
- Synthesis and Characterization of a Novel Photocleavable Fluorescent Dye Dyad for Diffusion Imaging.Chemical & biomedical imaging · 2025Article
- Cellular mechanisms of traumatic brain injury.npj biological physics and mechanics · 2025Review
- CHC22 clathrin recruitment to the early secretory pathway requires two-site interaction with SNX5 and p115.The EMBO journal · 2024Article
- Bioprinting of Synthetic Cell-like Lipid Vesicles to Augment the Functionality of Tissues after Manufacturing.ACS synthetic biology · 2024Article
- Engineering Tunable, Low Latency Spatial Computation with Dual Input Quorum Sensing Promoters.ACS synthetic biology · 2024Article
- Polysaccharide functionalization reduces lipid vesicle stiffness.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Experimentally Probing the Effect of Confinement Geometry on Lipid Diffusion.The journal of physical chemistry. B · 2024Article
- Bioorthogonal Metabolic Labeling of the Virulence Factor Phenolic Glycolipid in Mycobacteria.ACS chemical biology · 2024Article
- Kinetic study of membrane protein interactions: from three to two dimensions.Scientific reports · 2024Article
- What's past is prologue: FRAP keeps delivering 50 years later.Biophysical journal · 2023Review
- Using the Water Absorption Ability of Dried Hydrogels to Form Hydrogel-Supported Lipid Bilayers.Gels (Basel, Switzerland) · 2023Article
- Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer.Nature methods · 2023Article
- Transient interactions drive the lateral clustering of cadherin-23 on membrane.Communications biology · 2023Article
- Membrane Models and Experiments Suitable for Studies of the Cholesterol Bilayer Domains.Membranes · 2023Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence recovery after photobleaching (FRAP) is a standard method used to study the dynamics of lipids and proteins in artificial and cellular membrane systems. The advent of confocal microscopy two decades ago has made quantitative FRAP easily available to most laboratories. Usually, a single bleaching pattern/area is used and the corresponding recovery time is assumed to directly provide a diffusion coefficient, although this is only true in the case of unrestricted Brownian motion. Here, we propose some general guidelines to perform FRAP experiments under a confocal microscope with different bleaching patterns and area, allowing the experimentalist to establish whether the molecules undergo Brownian motion (free diffusion) or whether they have restricted or directed movements. Using in silico simulations of FRAP measurements, we further indicate the data acquisition criteria that have to be verified in order to obtain accurate values for the diffusion coefficient and to be able to distinguish between different diffusive species. Using this approach, we compare the behavior of lipids in three different membrane platforms (supported lipid bilayers, giant liposomes and sponge phases), and we demonstrate that FRAP measurements are consistent with results obtained using other techniques such as Fluorescence Correlation Spectroscopy (FCS) or Single Particle Tracking (SPT). Finally, we apply this method to show that the presence of the synaptic protein Munc18-1 inhibits the interaction between the synaptic vesicle SNARE protein, VAMP2, and its partner from the plasma membrane, Syn1A.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.