ArticlePLoS neglected tropical diseases2016
Molecular Characterization of Cryptosporidium Species and Giardia duodenalis from Symptomatic Cambodian Children.
Article in PLoS neglected tropical diseases, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 5 of them syntheses that pooled it.
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Who cites it
35 citing papers in PubMed, 5 syntheses or guidelines pooled it.
- Global prevalence ofParasitology · 2023Pooled it
- Pooled it
- Assessing the Impact of Animal Husbandry and Capture on Anemia among Women and Children in Low- and Middle-Income Countries: A Systematic Review.Advances in nutrition (Bethesda, Md.) · 2019Pooled it
- Anthroponotic transmission of Cryptosporidium parvum predominates in countries with poorer sanitation: a systematic review and meta-analysis.Parasites & vectors · 2019Pooled it
- Exposure to Animal Feces and Human Health: A Systematic Review and Proposed Research Priorities.Environmental science & technology · 2017Pooled it
- Silent shedding of zoonotic enteric protozoa in domestic pigs for human consumption in selected farms in the Iberian Peninsula.Veterinary research communications · 2026Article
- A pre-post pilot study with and without subsidy to promote improved backyard poultry-raising practices to reduce exposure to poultry and poultry feces in rural Bangladesh.PLOS global public health · 2026Article
- Cryptosporidium Diagnostic Assays: Molecular Detection and Species Determination by Real-Time PCR-Based Assays.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Prevalence and molecular characterization ofFood and waterborne parasitology · 2025Article
- Molecular identification and diversity of gastrointestinal apicomplexan protozoa in pigs in the Republic of Korea.Scientific reports · 2025Article
- Review
- Enteric Pathogens in Humans, Domesticated Animals, and Drinking Water in a Low-Income Urban Area of Nairobi, Kenya.Environmental science & technology · 2024Article
- Food and Waterborne Cryptosporidiosis from a One Health Perspective: A Comprehensive Review.Animals : an open access journal from MDPI · 2024Review
- Occurrence rate and species and subtypes of Cryptosporidium spp. in pet dogs in Yunnan Province, China.BMC microbiology · 2024Article
- Molecular characterization and zoonotic potential of Cryptosporidium spp. and Giardia duodenalis in humans and domestic animals in Heilongjiang Province, China.Parasites & vectors · 2024Article
- Prevalence of Intestinal Parasitic Infections, Genotypes, and Drug Susceptibility ofTropical medicine and infectious disease · 2023Article
- Review
- Article
- A survey ofPeerJ · 2022Article
- An Update on ZoonoticAnimals : an open access journal from MDPI · 2021Review
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Authors and funding
15 authors.
Funding
Abstract
backgroundIn a prospective study, 498 single faecal samples from children aged under 16 years attending an outpatient clinic in the Angkor Hospital for Children, northwest Cambodia, were examined for Cryptosporidium oocysts and Giardia cysts using microscopy and molecular assays. METHODOLOGY/PRINCIPAL
findingsCryptosporidium oocysts were detected in 2.2% (11/498) of samples using microscopy and in 7.7% (38/498) with molecular tests. Giardia duodenalis cysts were detected in 18.9% (94/498) by microscopy and 27.7% (138/498) by molecular tests; 82% of the positive samples (by either method) were from children aged 1-10 years. Cryptosporidium hominis was the most common species of Cryptosporidium, detected in 13 (34.2%) samples, followed by Cryptosporidium meleagridis in 9 (23.7%), Cryptosporidium parvum in 8 (21.1%), Cryptosporidium canis in 5 (13.2%), and Cryptosporidium suis and Cryptosporidium ubiquitum in one sample each. Cryptosporidium hominis and C. parvum positive samples were subtyped by sequencing the GP60 gene: C. hominis IaA16R6 and C. parvum IIeA7G1 were the most abundant subtypes. Giardia duodenalis was typed using a multiplex real-time PCR targeting assemblages A and B. Assemblage B (106; 76.8% of all Giardia positive samples) was most common followed by A (12.3%) and mixed infections (5.1%). Risk factors associated with Cryptosporidium were malnutrition (AOR 9.63, 95% CI 1.67-55.46), chronic medical diagnoses (AOR 4.51, 95% CI 1.79-11.34) and the presence of birds in the household (AOR 2.99, 95% CI 1.16-7.73); specifically C. hominis (p = 0.03) and C. meleagridis (p<0.001) were associated with the presence of birds. The use of soap was protective against Giardia infection (OR 0.74, 95% CI 0.58-0.95). CONCLUSIONS/SIGNIFICANCE: This is the first report to describe the different Cryptosporidium species and subtypes and Giardia duodenalis assemblages in Cambodian children. The variety of Cryptosporidium species detected indicates both anthroponotic and zoonotic transmission in this population. Interventions to improve sanitation, increase hand washing after defecation and before preparing food and promote drinking boiled water may reduce the burden of these two parasites.
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