Evidence map›Paper›PMID 27387517›Full record

ArticleCurrent genetics2017

HAL2 overexpression induces iron acquisition in bdf1Δ cells and enhances their salt resistance.

Lei Chen, Mingpeng Wang, Jin Hou, Jiafang Fu, Yu Shen, Fanghua Liu, Zhaojie Zhang, Xiaoming Bao

Open access · greenAbstract read
PubMed Publisher
In one paragraph

Article in Current genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Lei ChenState Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, China.
Mingpeng WangState Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, China.
Jin HouState Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, China.
Jiafang FuState Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, China.
Yu ShenState Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, China.
Fanghua LiuYantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, Shandong, 264003, China.
Zhaojie ZhangDepartment of Zoology and Physiology, University of Wyoming, Laramie, WY, USA.
Xiaoming BaoState Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, China. bxm@sdu.edu.cn.
Shandong University · CNState Key Laboratory of Microbial TechnologyUniversity of Wyoming · USYantai Institute of Coastal Zone Research · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The yeast Saccharomyces cerevisiae is capable of responding to various environmental stresses, such as salt stress. Such responses require a complex network and adjustment of the gene expression network. The goal of this study is to further understand the molecular mechanism of salt stress response in yeast, especially the molecular mechanism related to genes BDF1 and HAL2. The Bromodomain Factor 1 (Bdf1p) is a transcriptional regulator, which is part of the basal transcription factor TFIID. Cells lacking Bdf1p are salt sensitive with an abnormal mitochondrial function. We previously reported that the overexpression of HAL2 or deletion of HDA1 lowers the salt sensitivity of bdf1Δ. To better understand the mechanism behind the HAL2-related response to salt stress, we compared three global transcriptional profiles (bdf1Δ vs WT, bdf1Δ + HAL2 vs bdf1Δ, and bdf1Δhda1Δ vs bdf1Δ) in response to salt stress using DNA microarrays. Our results reveal that genes for iron acquisition and cellular and mitochondrial remodeling are induced by HAL2. Overexpression of HAL2 decreases the concentration of nitric oxide. Mitochondrial iron-sulfur cluster (ISC) assembly also decreases in bdf1Δ + HAL2. These changes are similar to the changes of transcriptional profiles induced by iron starvation. Taken together, our data suggest that mitochondrial functions and iron homeostasis play an important role in bdf1Δ-induced salt sensitivity and salt stress response in yeast.

Indexed as

Bromodomain Containing ProteinsGene Expression ProfilingGene Expression Regulation, FungalGene OntologyImmunoblottingIronMutationNucleotidasesOligonucleotide Array Sequence AnalysisReverse Transcriptase Polymerase Chain ReactionSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSalt ToleranceSignal TransductionSodium ChlorideStress, PhysiologicalBDF1 protein, S cerevisiaebisphosphoadenylate 3'-nucleotidaseBromodomain Containing ProteinsIronNucleotidasesSaccharomyces cerevisiae ProteinsSodium ChlorideTranscription FactorsBromodomain factor 1HAL2HDA1Iron acquisitionSalt stress response

Identifiers

PMID27387517
OpenAlexW2460867174

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.