Evidence map›Paper›PMID 27387482›Full record

ArticleEndocrinology2016

Gq Protein-Coupled Membrane-Initiated Estrogen Signaling Rapidly Excites Corticotropin-Releasing Hormone Neurons in the Hypothalamic Paraventricular Nucleus in Female Mice.

Pu Hu, Ji Liu, Ali Yasrebi, Juliet D Gotthardt, Nicholas T Bello, Zhiping P Pang, Troy A Roepke

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Endocrinology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
2.0field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 40 citations in OpenAlex.

  1. Review
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  9. Sex differences in autonomic responses to stress: implications for cardiometabolic physiology.American journal of physiology. Endocrinology and metabolism · 2022
    Review
  10. Review
  11. Review
  12. Sex-Related Predisposition to Post-Traumatic Stress Disorder Development-The Role of Neuropeptides.International journal of environmental research and public health · 2021
    Review
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Role of estrogen and stress on the brain-gut axis.American journal of physiology. Gastrointestinal and liver physiology · 2019
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Pu HuDepartment of Animal Sciences (P.H., A.Y., J.D.G., N.T.B., T.A.R.), School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, and Child Health Institute of New Jersey (J.L., Z.P.P.) and Department of Neuroscience and Cell Biology (J.L., Z.P.P.), Rutgers Robert Wood Johnson Medical School, New Brunswick, New Jersey 08901.
Ji LiuDepartment of Animal Sciences (P.H., A.Y., J.D.G., N.T.B., T.A.R.), School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, and Child Health Institute of New Jersey (J.L., Z.P.P.) and Department of Neuroscience and Cell Biology (J.L., Z.P.P.), Rutgers Robert Wood Johnson Medical School, New Brunswick, New Jersey 08901.
Ali YasrebiDepartment of Animal Sciences (P.H., A.Y., J.D.G., N.T.B., T.A.R.), School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, and Child Health Institute of New Jersey (J.L., Z.P.P.) and Department of Neuroscience and Cell Biology (J.L., Z.P.P.), Rutgers Robert Wood Johnson Medical School, New Brunswick, New Jersey 08901.
Juliet D GotthardtDepartment of Animal Sciences (P.H., A.Y., J.D.G., N.T.B., T.A.R.), School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, and Child Health Institute of New Jersey (J.L., Z.P.P.) and Department of Neuroscience and Cell Biology (J.L., Z.P.P.), Rutgers Robert Wood Johnson Medical School, New Brunswick, New Jersey 08901.
Nicholas T BelloDepartment of Animal Sciences (P.H., A.Y., J.D.G., N.T.B., T.A.R.), School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, and Child Health Institute of New Jersey (J.L., Z.P.P.) and Department of Neuroscience and Cell Biology (J.L., Z.P.P.), Rutgers Robert Wood Johnson Medical School, New Brunswick, New Jersey 08901.
Zhiping P PangDepartment of Animal Sciences (P.H., A.Y., J.D.G., N.T.B., T.A.R.), School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, and Child Health Institute of New Jersey (J.L., Z.P.P.) and Department of Neuroscience and Cell Biology (J.L., Z.P.P.), Rutgers Robert Wood Johnson Medical School, New Brunswick, New Jersey 08901.
Troy A RoepkeDepartment of Animal Sciences (P.H., A.Y., J.D.G., N.T.B., T.A.R.), School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, and Child Health Institute of New Jersey (J.L., Z.P.P.) and Department of Neuroscience and Cell Biology (J.L., Z.P.P.), Rutgers Robert Wood Johnson Medical School, New Brunswick, New Jersey 08901.
Rutgers, The State University of New Jersey · US

Funding

Translational Research Support CoreP30ES005022 · NIEHS · UNIV OF MED/DENT NJ-R W JOHNSON MED SCH · PI PANOS G. GEORGOPOULOS · 1988 to 2026
$47.4M
Estrogen Regulation of Channels Involved in the Control of Energy HomeostatisR00DK083457 · NIDDK · RUTGERS, THE STATE UNIV OF N.J. · PI ROEPKE, TROY ADAM · 2011 to 2014
$925k
NIDDK NIH HHS R00 DK083457NIEHS NIH HHS P30 ES005022
6 · The paper itself

Abstract

CRH neurons in the hypothalamic paraventricular nucleus (PVN) play a central role in regulating the hypothalamus-pituitary-adrenal (HPA) axis and are directly influenced by 17β-estradiol (E2). Although compelling evidence has suggested the existence of membrane-associated estrogen receptors (mERs) in hypothalamic and other central nervous system neurons, it remains unknown whether E2 impacts CRH neuronal excitability through this mechanism. The purpose of the current study is to examine the existence and function of mER signaling in PVN CRH neurons. Whole-cell recordings were made from CRH neurons identified by Alexa Fluor 594 labeling and post hoc immunostaining in ovariectomized female mice. E2 (100nM) rapidly suppressed the M-current (a voltage-dependent K(+) current) and potentiated glutamatergic excitatory postsynaptic currents. The putative Gq-coupled mER (Gq-mER) characterized in hypothalamic proopiomelanocortin neurons initiates a phospholipase C-protein kinase C-protein kinase A pathway; therefore, we examined the involvement of this pathway using selective inhibitors. Indeed, the ER antagonist ICI 182780 and inhibitors of Gq-phospholipase C-protein kinase C-protein kinase A blocked E2's actions, suggesting dependence on the Gq-mER. Furthermore, STX, a selective ligand for the Gq-mER, mimicked E2's actions. Finally, to examine the in vivo effect of Gq-mER activation, E2 or STX injection increased c-fos expression in CRH neurons in the PVN, suggesting CRH neuronal activation. This corresponded to an increase in plasma corticosterone. We conclude that the Gq-mER plays a critical role in the rapid regulation of CRH neuronal activity and the HPA axis. Our findings provide a potential underlying mechanism for E2's involvement in the pathophysiology of HPA-associated mood disorders.

Indexed as

Corticotropin-Releasing HormoneAcrylamidesAnilidesAnimalsCyclic AMP-Dependent Protein KinasesEstradiolExcitatory Postsynaptic PotentialsFemaleGTP-Binding Protein alpha Subunits, Gq-G11Mice, Inbred C57BLMice, KnockoutNeuronsNitrilesParaventricular Hypothalamic NucleusPropionatesProtein Kinase C-delta2,3-bis(4-hydroxyphenyl)-propionitrile2-(4-hydroxyphenyl)-3-phenylpent-2-enoic acid (4-(2-dimethylaminoethoxy)phenyl)amideAcrylamidesAnilidesCorticotropin-Releasing HormoneCyclic AMP-Dependent Protein KinasesEstradiolGTP-Binding Protein alpha Subunits, Gq-G11NitrilesPropionatesProtein Kinase C-deltaType C Phospholipases

Identifiers

PMID27387482
PMCPMC5007888
OpenAlexW2470901702

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.