ArticleMicrobial cell factories2016
The pivotal role of malic enzyme in enhancing oil accumulation in green microalga Chlorella pyrenoidosa.
Article in Microbial cell factories, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 78 citations in OpenAlex.
- Unraveling metabolism underpinning biomass composition shift inFrontiers in plant science · 2025Article
- Role of Cytosolic Malic Enzyme in Oleaginicity of High-Lipid-Producing Fungal StrainJournal of fungi (Basel, Switzerland) · 2022Article
- Mucor circinelloides: a model organism for oleaginous fungi and its potential applications in bioactive lipid production.Microbial cell factories · 2022Review
- Overexpression of the Mitochondrial Malic Enzyme Genes (Frontiers in microbiology · 2022Article
- Metabolomics and lipid profile analysis of Coccomyxa melkonianii SCCA 048.Extremophiles : life under extreme conditions · 2021Article
- Establishment of a Genome Editing Tool Using CRISPR-Cas9 inInternational journal of molecular sciences · 2021Article
- The establishment of new protein expression system using N starvation inducible promoters in Chlorella.Scientific reports · 2020Article
- Interorganelle Communication: Peroxisomal MALATE DEHYDROGENASE2 Connects Lipid Catabolism to Photosynthesis through Redox Coupling in Chlamydomonas.The Plant cell · 2018Article
- High-efficiency promoter-driven coordinated regulation of multiple metabolic nodes elevates lipid accumulation in the model microalga Phaeodactylum tricornutum.Microbial cell factories · 2018Article
- The Role of Malic Enzyme on Promoting Total Lipid and Fatty Acid Production inFrontiers in plant science · 2018Article
- Time-resolved transcriptome analysis and lipid pathway reconstruction of the oleaginous green microalgaBiotechnology for biofuels · 2017Article
- The dilemma for lipid productivity in green microalgae: importance of substrate provision in improving oil yield without sacrificing growth.Biotechnology for biofuels · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe fast growing photosynthetic microalgae have been widely used in aquaculture, food, health, and biofuels. Recent findings in the diatom has proposed a pivotal role of NADP-malic enzyme in generation of NADPH as an important supply of reducing power for fatty acid biosynthesis. To test the lipogenic malic enzyme for fatty acid synthesis in green algae, here the malic enzyme gene PtME from the oleaginous diatom Phaeodactylum tricornutum was expressed in a representative green microalga Chlorella pyrenoidosa.
resultsThe engineered C. pyrenoidosa strain showed higher enzymatic activity of malic enzyme which subsequently promoted fatty acid synthesis. The neutral lipid content was significantly increased by up to 3.2-fold than wild type determined by Nile red staining, and total lipid content reached 40.9 % (dry cell weight). The engineered strain exhibited further lipid accumulation subjected to nitrogen deprivation condition. Upon nitrogen deprivation, engineered microalgae accumulated total lipid up to 58.7 % (dry cell weight), a 4.6-fold increase over the wild type cells under normal culture condition. At cellular level, increased volume and number of oil bodies were observed in the engineered microalgal cells.
conclusionsThese findings suggested that malic enzyme is a pivotal regulator in lipid accumulation in green microalga C. pyrenoidosa, and presenting a breakthrough of generating ideal algal strains for algal nutrition and biofuels.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.